イーストのGTP結合タンパク質YPT1と哺乳類の同類は,分泌機構と関連しています
N Segev1, J Mulholland, D Botstein
1Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
Cell
|March 25, 1988
まとめ
イーストのGTP結合タンパク質であるYPT1遺伝子製品は,初期の分泌経路において重要な役割を果たしています. このタンパク質は,
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- バイオケミストリー バイオケミストリー
背景:
- YPT1遺伝子産物は,酵母によるGTP結合タンパク質で,細胞輸送に関与している.
- YPT1の変異は,早期の分泌経路を混乱させ,膜と膀の蓄積につながります.
研究 の 目的:
- 酵母YPT1遺伝子産物の分泌経路における機能を調査する.
- YPT1タンパク質とその哺乳類の同位体の局所を決定する.
主な方法:
- 酵母遺伝学と突然変異分析 (ypt1-1変異).
- YPT1.1.1に対する親和性浄化抗体を用いた免疫光顕微鏡検査
- 分泌とグリコシライゼーションの欠陥 (インバーターゼ) の分析.
- 哺乳類の細胞染色 (小麦芽アグルチニン) と比較.
主要な成果:
- ypt1-1変異は,早期の分泌欠陥変異体をフェノコピーし,膜/膀の蓄積と分泌欠陥を引き起こす.
- YPT1タンパク質はサイトプラズマに局所化し,酵母芽に強烈に結合する.
- 異種変異体における異常なゴルギ構造は,YPT1の染色パターンを変化させる.
- 哺乳類のYPT1の同類は,小麦の芽アグルチニンに類似して,ゴルギ器官に局所する.
結論:
- イーストのGTP結合タンパク質,YPT1のようなものは,細胞内膀の伝達を指揮する上で極めて重要です.
- YPT1タンパク質とその哺乳類の同類体は,分泌経路の初期段階に関与しています.
- 研究結果は,GTP結合タンパク質が,種間の膀輸送において,保存された役割を担うことを示唆している.
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