成長と分化の間,そして細胞の脱極化後に,c-fosと共同調節された亜鉛指をコードする遺伝子

V P Sukhatme¹, X M Cao, L C Chang

¹Department of Medicine, Howard Hughes Medical Institute, University of Chicago, Illinois 60637.

Cell

|April 8, 1988

まとめ

早期成長応答タンパク質1 (Egr-1) は,転写調節体として作用する. この遺伝子は,様々な細胞で急速な誘導を示し,生物学的プロセスにおける重要な役割を果たしていることを示唆しています.

科学分野:

分子生物学は分子生物学である.
遺伝学遺伝学とは
細胞生物学細胞生物学

背景:

早期成長応答タンパク質1 (Egr-1) は,すぐ早期の遺伝子である.
Egr-1は,ミトゲン刺激への反応として,c-fosに類似した急速誘導運動を示している.
Egr-1は,様々な刺激に対する細胞の反応に役割を果たします.

研究の目的:

Egr-1遺伝子の特徴と潜在的な機能を調査する.
Egr-1.の配列とゲノム位置を分析する.
細胞の分化と活性化中のEGR-1の発現パターンを理解する.

主な方法:

ネズミのEGR-1cDNAの配列分析.
人間のEGR1遺伝子の遺伝子マッピング.
Egr-1 mRNAの発現レベルを様々な細胞タイプと状態 (in vitroとin vivo) で分析する.

主要な成果:

配列解析は,DNAを結合する3本の亜鉛指を持つタンパク質を予測した.
人間のEGR1遺伝子は,染色体5 (帯5q23-31) に位置しています.
Egr-1 mRNAレベルは,心臓および神経細胞の微分化および膜脱極化後に有意に増加します.

結論:

Egr-1は,転写調節体として機能する.
Egr-1はc-fosと共調されており,共通の規制経路を示しています.
Egr-1は,細胞の分化と刺激への反応を含む多様な生物学的プロセスに関与しています.

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