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プロト脊椎動物の包括的な単細胞トランスクリプトーム系統
Chen Cao1, Laurence A Lemaire1, Wei Wang2
1Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ, USA.
Nature
|July 12, 2019
まとめ
この研究は,アシディアン胚の 9万以上の単細胞トランスクリプトームをマッピングし,細胞系統と神経サブタイプを明らかにします. これらの発見は,テレンセファロンを含む脊椎動物の細胞の進化的起源を明らかにします.
科学分野:
- 発達生物学
- 進化生物学
- ゲノミクス
背景:
- アシディアン胚は動物の発達と脊椎動物の細胞種の進化を理解する上で貴重なモデルです.
- アシディアのような原脊椎動物は,頭蓋骨のプラコードや 神経頂部細胞などの複雑な構造の起源についての洞察を提供します.
研究 の 目的:
- ガストルレーションから泳ぐタドウの段階までのアシディアンの発達に関する包括的な単細胞トランスクリプトームアトラスを生成する.
- 幼虫の神経サブタイプのための仮想細胞系地図と遺伝子ネットワークを構築する.
- 脊椎動物細胞の進化的起源をアシディアン発達のデータを使って追跡する.
主な方法:
- シオナ・インテスティナリスの胚から9万以上の細胞の単細胞RNA配列解析
- 細胞系図を構築するためのトランスクリプトーム軌道の計算分析.
- 幼虫の神経系内の41の神経サブタイプの識別と注釈.
主要な成果:
- すべての発達段階と細胞タイプをカバーする高解像度のトランスクリプトームデータ.
- 神経発達のための仮想細胞系図と暫定的な遺伝子規制ネットワークの構築.
- スイミングタッドポールのシナプトームと脊椎動物の脳構造との進化的リンクの識別.
結論:
- 生成されたデータセットは,アシディアンの発達と進化を研究するための前例のないリソースを提供します.
- この研究は,テレンセファロンを含む重要な脊椎動物細胞の進化軌道を明らかにしています.
- この研究は,単細胞トランスクリプトミクスが発達過程と進化史を再構築する力を示しています.
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