結晶化を制御するための新しい添加物として,ウビキチン設計者タンパク質

Cristina Ruiz-Agudo¹, Joachim Lutz¹, Philipp Keckeis¹

¹Department of Chemistry , University of Konstanz , 78457 Konstanz , Germany.

Journal of the American Chemical Society

|July 20, 2019

まとめ

特定の鉱物相互作用機能を備えた遺伝子工学によるユビキチンタンパク質は鉱化を制御できますこの新しい設計タンパク質は結晶の形成と形状をプログラム可能な制御を可能にします

科学分野:

バイオマテリアル科学
タンパク質工学
結晶化科学

背景:

生物鉱物化には様々なタンパク質が含まれますが,鉱物形成に対する合成制御は困難です.
ミネラルと相互作用する機能の特定の3Dアレンジメントを持つバイオインスパイアされたマクロモлекуルを設計することは困難です.
表面機能の配置が鉱化にどのように影響するかを理解するのは難しい.

研究の目的:

制御された鉱化のための特定の鉱物相互作用機能を持つユビキチン (Ub) タンパク質を設計する.
鉱化過程でタンパク質の表面に定義された3D配置の影響を調査する.
バイオインスピレーションの付加物の限界を克服する

主な方法:

遺伝子組み換えウビキチン (Ub) タンパク質
非自然なアミノ酸とミネラル相互作用機能を選択的圧力組み込みとクリック化学で導入する.
機能化中のUb二次構造の保存

主要な成果:

定義された位置で3つのリン酸機能を持つUBタンパク質は,ユニークな鉱化制御を示した.
肉眼で見える密度の高い液体鉱物相フィルムの安定化.
鉱物相を無形なナノ粒子と複雑な形状の結晶に変換する.

結論:

Ub設計タンパク質は新世代の結晶化添加物を表しています.
Ubの鉱物相互作用機能の3D配置はプログラム可能で設計できます.
これらの設計タンパク質は,ターゲット指向の鉱化制御の可能性を提供します.

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