Pol II リン酸化は,トランスクリプションとスプライシングコンデンサートの間の切り替えを調節する
Yang Eric Guo1, John C Manteiga1,2, Jonathan E Henninger1
1Whitehead Institute for Biomedical Research, Cambridge, MA, USA.
Nature
|August 9, 2019
まとめ
RNAポリメラーゼIIのリン酸化
科学分野:
- 分子生物学
- 細胞生物学
- 生物化学
背景:
- RNAポリメラーゼII (Pol II) C末端ドメインのリン酸化は,転写開始および延長に不可欠である.
- トランスクリプションとスプライシング装置は相分離コンデンサを形成し,Pol IIやスプライシングファクターのような分子を集約します.
- Pol II C末端ドメインのリン酸化効果に関する既存のモデルは不完全である.
研究 の 目的:
- Pol II C末端ドメインのリン酸化が相分離凝縮物への組み込みを調節するかどうかを調査する.
- トランスクリプションとスプライシングコンデンサートの間のダイナミックな交換におけるリン酸化の役割を決定する.
主な方法:
- 低酸化および高酸化Pol II C末端ドメインを相分離コンデンサに組み込むことを調査した.
- 媒介体とスプライシングファクター凝縮物とのPol II C端域相互作用を観察するために生化学的測定を用いた.
主要な成果:
- 低酸化したPol II C末端ドメインは媒介体凝縮物に組み込まれている.
- サイクリン依存キナーゼのリン酸化は,Pol IIを媒介体コンデンサートに組み込むのを減少させる.
- ハイパーフォスホル化したPol II C末端領域は,スプライシング因子濃縮物への好ましい組み込みを示している.
結論:
- Pol II C末端ドメインのリン酸化により,転写開始コンデンサからRNA処理コンデンサへの切り替えが行われます.
- リン酸化は,Pol IIのコンデンサート偏好を制御する重要な規制メカニズムとして機能します.
- このリン酸化依存のコンデンサート交換は,転写開始とRNA処理を結びつける.
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