トリパノソーマ・クルジは,哺乳類の上皮細胞を偏光的に侵入する

S Schenkman¹, N W Andrews, V Nussenzweig

¹Department of Pathology, New York University Medical Center, New York 10016.

Cell

|October 7, 1988

まとめ

細胞の極性は寄生虫の侵入に影響する. トライパノソーマ・クルージは,主細胞をベース・ラテラル・ドメインで感染し,偏光化が少ない細胞は感染率が高い.

科学分野:

細胞生物学細胞生物学
寄生虫学とは,寄生虫学である.
感染症感染症は感染症です.

背景:

ホスト細胞の極性性は,病原体の侵入と感染症の動態における重要な要因です.
チャガス病の原因であるトライパノソーマ・クルージは,複雑な宿主-寄生虫の相互作用を示しています.
寄生虫の侵入メカニズムを理解することは,効果的な疾病管理戦略の開発に不可欠です.

研究の目的:

トライパノソーマ・クルージによる感染プロセスにおける宿主細胞の極性性の役割を調査する.
トライパノソーマ・クルージの特定のエントリーポイントを極化性上皮細胞に決定する.

主な方法:

マディン・ダービー犬腎臓 (MDCK) の上皮細胞を活用し,極化細胞のモデルとなった.
寄生虫感染の定量的な評価のためにDNAプローブを使用しました.
スキャン電子顕微鏡を用いて,寄生虫の侵入部位を視覚化しました.

主要な成果:

流動的,極化MDCK細胞は,Trypanosoma cruziがベースラテラルドメインを通って優遇的に侵入することを示しました.
分極化が弱く,薄板状のMDCK細胞は,感染に対する感受性が高かった.
スキャニング電子顕微鏡では,寄生虫の約92%が細胞の縁から侵入することを明らかにしました.

結論:

宿主細胞の極性性は,トライパノソーマ・クルージの侵入に大きく影響する.
ベースラテラル領域は,Trypanosoma cruziのメタサイクリックおよびトリポマスティゴート段階の好ましいエントリーサイトです.
寄生虫の侵入は空間的に調節され,極化細胞の細胞縁を好む傾向が高い.

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