関連する実験動画
Updated: Jan 21, 2026
07:11
Constructing Cyclic Peptides Using an On-Tether Sulfonium Center
Published on: September 28, 2022
3.1K
サイクルピロール・イミダゾールポリアミドの前向き/逆向きの偏好の制御
Yuki Hirose1, Sefan Asamitsu1, Toshikazu Bando1
1Department of Chemistry, Graduate School of Science , Kyoto University , Sakyo , Kyoto 606-8502 , Japan.
Journal of the American Chemical Society
|August 10, 2019
まとめ
ピロルイミダゾールポリアミド (PIPs) はDNAを結合することができる. 周期性PIP (cPIP) のアミノ群の位置を変更すると,そのDNA結合方向が制御され,標的DNA配列の選択が強化されます.
科学分野:
- 分子生物学
- 生物化学
- 有機化学
背景:
- ピロルイミダゾールポリアミド (PIP) は,特定のDNA配列に結合する分子である.
- PIPはDNAを前向きに (5'-3'/N-C) または逆向きに (5'-3'/C-N) 結合することができる.
- 逆結合は意図しない相互作用につながる可能性があります.
研究 の 目的:
- サイクルPIP (cPIP) の光学または構造的同位体合成および調査.
- cPIPの改変がDNA結合方向にどのように影響するかを決定する.
- PIPのDNA結合特異性を制御する方法を探求する.
主な方法:
- γ-ターン単位で異なるアミノ群の位置を持つ周期的なPIPイソマーの合成.
- 前向きと逆向きの両方の結合アフィニティの調査.
- 異なるcPIP同位体とヘアピンPIPの間の結合偏好の比較
主要な成果:
- (R) -α-アミノ-γ-ターンユニットを持つcPIPは,前向きに結合する方向性を好みます.
- (S) -α-アミノ-γ-ターンを持つcPIPは,逆の結合方向を好みます.
- γ-ターン単位におけるアミノ群の位置が,cPIPの偏向を決定する.
結論:
- cPIPの方向偏りは, γ-ターンユニットのアミノ群の立体化学を変化させることで正確に制御できます.
- 結合指向に対するこの制御は,PIPによって標的となるDNA配列の範囲を大幅に拡大する.
- 発見により より特定で多用途な DNA 標的薬の開発が進められます
関連する概念動画
02:33Stereoisomerism of Cyclic Compounds
11.0K
In this lesson, we delve into the role of ring conformation and its stability, which determines the spatial arrangement and, consequently, the molecular symmetry and stereoisomerism of cyclic compounds. 1,2-Dimethylcyclohexane is used as a case study to evaluate the possible number of stereoisomers. Here, given the multiple (n = 2) chiral centers, there are 2n = 4 possible configurations that lack a plane of symmetry, as the ring skeleton exists in a non-planar chair conformation. In addition,...
11.0K
01:13Location and Orientation of the Heart
9.3K
The human heart, despite its modest size and weight, is an organ of remarkable strength and endurance. Roughly the size of a fist, the heart weighs between 250 and 350 grams and is nestled within the mediastinum, the medial cavity of the thorax. It extends obliquely for about 12 to 14 cm, resting on the superior surface of the diaphragm. The heart is positioned anterior to the vertebral column and posterior to the sternum, with two-thirds of its mass lying to the left of the midsternal line.
9.3K
01:19Cyclic Processes And Isolated Systems
3.4K
A thermodynamic system with zero heat exchange and work is an isolated system. For these systems, the internal energy remains constant.
In the case of a non-isolated system, the change in the internal energy is zero only if the process is cyclic. A thermodynamic process is considered cyclic if the system undergoes a series of changes and returns to its initial state.
Consider a cyclic process that returns to its initial state, undergoing a four-step process. The heat transfer along each...
In the case of a non-isolated system, the change in the internal energy is zero only if the process is cyclic. A thermodynamic process is considered cyclic if the system undergoes a series of changes and returns to its initial state.
Consider a cyclic process that returns to its initial state, undergoing a four-step process. The heat transfer along each...
3.4K
01:10Voltammetric Techniques: Cyclic Voltammetry
1.5K
Cyclic voltammetry (CV) is an electrochemical technique used to investigate the redox properties of a chemical species. It involves measuring the current response of an electrochemical cell as a function of the applied potential. The setup for cyclic voltammetry typically consists of a working electrode, a reference electrode, and a counter electrode—all immersed in an electrolyte solution. The working electrode is where the redox reaction of interest occurs, while the reference electrode...
1.5K
01:14Diode: Reverse bias
1.8K
A diode is reverse-biased when the positive terminal of an external voltage source is connected to the n-type material and the negative terminal to the p-type material. This configuration opposes the natural direction of current flow through the diode, effectively increasing the width of the depletion region and the barrier potential. The reverse bias condition produces a minimal leakage current, primarily due to minority charge carriers. This leakage becomes significant when the reverse...
1.8K
01:14Reversible and Irreversible Processes
5.6K
The thermodynamic processes can be classified into reversible and irreversible processes. The processes that can be restored to their initial state are called reversible processes. It is only possible if the process is in quasi-static equilibrium, i.e., it takes place in infinitesimally small steps, and the system remains at equilibrium However, these are ideal processes and do not occur naturally. An ideal system undergoing a reversible process is always in thermodynamic equilibrium within...
5.6K