モルフォジェネティック波の遺伝的誘導と機械化学的伝播
Anaïs Bailles1,2, Claudio Collinet3,4, Jean-Marc Philippe1,2
1IBDM-UMR7288, Aix Marseille Université and CNRS, Marseille, France.
Nature
|August 16, 2019
まとめ
ドロソフィラの内皮の発達中にアクトミオシン活性を制御する2つのモードがあります. 局所転写はミオシンII (MyoII) アクティベーションの波を起こすが,その波はメカニカルに細胞の侵入を促す.
科学分野:
- 発達生物学
- 細胞生物学
- バイオ物理学
背景:
- 組織形態化は,アクトミオシン収縮によって引き起こされる細胞の形状の変化に依存しています.
- 遺伝子発現パターンはアクトミオシン収縮性を制御し,細胞の行動を地域化する.
研究 の 目的:
- ドロソフィラの内皮形態変異過程でRho1とミオシンIIの活性化を制御するメカニズムを調査する.
- 組織規模の細胞の侵入を誘導する 転写と機械的なシグナル間の相互作用を解明する.
主な方法:
- ドロソフィラをモデル生物として利用した.
- Rho1とMyoIIの活性化パターンを調査した.
- 細胞の侵入におけるフォグ・リガンドとメカニカル・フィードバックの役割を調べた.
主要な成果:
- Rho1-MyoII活性化の2つのモードを特定した:局所転写開始と組織規模の波.
- 波は機械的にMyoIIによって駆動され 持続的転写やフォグによって 駆動されないことが示されました
- 一つの細胞列におけるMyoIIの活性化と陰化が,インテグリン経由で次の列における粘着と,その後の活性化を促進することを示した.
結論:
- ドロソフィラ内皮の形態変異は局所転写によって開始され,機械的に駆動されたフィードバックループによって拡散される.
- MyoIIの活動と細胞の変形は 細胞の連続的侵入と組織の形成を促すサイクルを生み出します
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