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関連する概念動画

RNA Splicing01:32

RNA Splicing

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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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Alternative RNA Splicing02:18

Alternative RNA Splicing

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Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
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Alternative RNA Splicing02:18

Alternative RNA Splicing

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The Thoracic Cage: Sternum01:17

The Thoracic Cage: Sternum

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The thoracic or rib cage forms the body's thorax (chest) portion. Its primary function in the body is to protect vital organs in the thoracic cavity, such as the heart and the lungs. It consists of 12 pairs of ribs with their costal cartilages and the sternum. The ribs are anchored posteriorly to the 12 thoracic vertebrae (T1-T12).
The sternum is the elongated bony structure on the anterior side of the thoracic cage. It consists of three parts: the manubrium, the body, and the xiphoid...
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The Thoracic Cage: Ribs01:20

The Thoracic Cage: Ribs

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Ribs are curved, flattened bones forming the thoracic cavity wall with the thoracic muscles. There are 12 pairs of thoracic ribs. The posterior ends of all the ribs articulate with the T1–T12 thoracic vertebrae. In contrast,the anterior ends of most ribs attach to the sternum via their costal cartilages.
Parts of a Typical Rib
A typical rib has a head, neck, and body. The posterior end of the rib is called the head, followed by a narrow neck. The head articulates primarily with the costal...
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¹H NMR: Complex Splitting01:13

¹H NMR: Complex Splitting

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A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied...
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Synthesis of an Intein-mediated Artificial Protein Hydrogel
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カージド・スプリット・インテインを用いた近接誘発スプライシング

Josef A Gramespacher1, Antony J Burton1, Luis F Guerra1

  • 1Department of Chemistry , Princeton University , Frick Laboratory, Princeton , New Jersey 08544 , United States.

Journal of the American Chemical Society
|August 17, 2019
PubMed
まとめ
この要約は機械生成です。

科学者は近接トリガーを用いて 条件付きタンパク質スプライシング (CPS) の新しい方法を開発しました この技術により 細胞内のタンパク質の活性化が 精密に制御され 生物学的研究と治療の応用に 新たな可能性が生まれます

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関連する実験動画

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科学分野:

  • 生物化学
  • 分子生物学
  • 細胞生物学

背景:

  • 自然に分裂したインテインは,ポリペプチド結合のためのタンパク質トランススプライシング (PTS) を促進する.
  • 条件付きタンパク質スプライシング (CPS) は,タンパク質機能の翻訳後の制御を可能にします.
  • CPSは時間的,空間的な タンパク質の活性化をもたらします

研究 の 目的:

  • Npu inteinを使用した最初の近接触発CPS方法を提示します.
  • この方法の汎用性を様々な近接トリガーで示します.
  • 哺乳類の細胞におけるアセチルトランスフェラーゼp300の局所的活性化を示す.

主な方法:

  • CPSのために自然に分割され,迅速なスプライシングのNPUインテインを使用しました.
  • スプライシングを始めるために様々な近接トリガーを開発した.
  • 標的タンパク質を再構成して活性化するために哺乳類の細胞にシステムを適用しました.

主要な成果:

  • NPUインテインを使って 接近誘発CPSを成功裏に実証しました
  • 複数の近接トリガーの 互換性を示した
  • 哺乳類の細胞でp300アセチルトランスフェラーゼの局所的活性化を達成した.

結論:

  • Npu inteinを使用した近接誘発CPSは実行可能な技術です.
  • この方法は,精密で局所的なタンパク質の活性化を可能にします.
  • 生物学的システムにおける タンパク質の機能を制御するための 新しい道を開きます