マイクロチューブル関連タンパク質MAP2は,タウタンパク質とマイクロチューブル結合モチーフを共有しています
S A Lewis1, D H Wang, N J Cowan
1Department of Biochemistry, New York University Medical Center, NY 10016.
Science (New York, N.Y.)
|November 11, 1988
まとめ
マイクロチューブル関連タンパク質2 (MAP2) は,そのカルボキシル末端領域にマイクロチューブルを結合するリピート領域を含んでいます. この発見は,タウタンパク質に似ているが,マイクロチューブル結合タンパク質の一般的なメカニズムを示唆している.
科学分野:
- 神経科学は神経科学である.
- 分子生物学は分子生物学である.
- バイオケミストリー バイオケミストリー
背景:
- マイクロチューブル関連タンパク質2 (MAP2) は,脳のマイクロチューブルの主要な成分です.
- MAP2は,アルツハイマー病の神経線維細胞の絡みの中に存在するタウタンパク質と抗原決定素を共有している.
研究 の 目的:
- マウスの脳の完全な配列を決定するには MAP2.2.
- MAP2のマイクロチューブル結合ドメインを特定する.
- MAP2-マイクロチューブル相互作用の構造的基礎を調査する.
主な方法:
- マウスの脳MAP2をコードする補完的なDNAのクローニングとシーケンシング.
- MAP2ポリペプチド断片のインビトロ発現.
- 顕微管共浄化試験は,発現したMAP2断片を用いて行われます.
主要な成果:
- MAP2のカルボキシル末端の185アミノ酸は,タウタンパク質と67%の類似性を示しています.
- この領域には3つの不完全な18アミノ酸の繰り返しがあります.
- これらの繰り返しを含む発現したMAP2断片はマイクロチューブルで共浄化されたが,アミノ末端断片はそうではなかった.
結論:
- 18アミノ酸の繰り返しを含むMAP2のカルボキシル末端ドメインは,マイクロチューブルの結合に責任があります.
- これらの繰り返しは,MAP2およびtauタンパク質におけるマイクロチューブル結合の保存された特徴を表している可能性があります.
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