微生物から派生したランチバイオティクスは,バンコミシン耐性エンテロコックスの耐性を回復させる
Sohn G Kim1,2, Simone Becattini1, Thomas U Moody1,3
1Immunology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, USA.
Nature
|August 23, 2019
まとめ
腸内細菌であるブルーティア・プロダクタBPSCSKは,バンコミシン耐性エンテロコッカス・フェシウム (VRE) のコロニー化を抑制するランチバイオティクスを産生する. VRE感染に対する 益生菌として有望です
科学分野:
- 微生物学
- 胃腸内科
- 感染症
背景:
- 腸内共生細菌は通常,ヴァンコミシン耐性エンテロコッカス・フェシウム (VRE) によるコロニー化を防ぐ.
- 抗生物質の使用は この障壁を壊し 病院で得られた感染症の一般的な原因である VRE への感受性を高めます
- Blautia producta BPSCSKを含む特定の共生コンソーシアムは,抗生物質誘発のVRE感受性を逆転させる可能性を示しています.
研究 の 目的:
- ブラウティア・プロダクタBPSCSKがVREコロニー化を阻害するメカニズムを調査する.
- VREに対するBPSCSKおよびその分泌されたランチバイオティックの有効性をin vitroおよびin vivoで比較する.
- VRE耐性に対するプロバイオティクスとしてのランチバイオティクスを産生するコンメンサルの可能性を調査する.
主な方法:
- VRE阻害のBPSCSKのメカニズムを調査し,分泌されたランチビオティックを特定した.
- BPSCSKとLactococcus lactisがVREの成長とコロニー化に及ぼすインビトロ効果とインビボ効果を比較した.
- 患者サンプルと無菌マウスのランチバイオティクスの遺伝子濃度との関連を分析した.
主要な成果:
- BPSCSKはニシンAに似たランチバイオティクスを分泌することでVREを阻害する.
- BPSCSKとL. lactisの両方がVREをインビトロ抑制するが,BPSCSKだけがVRE密度をインビボ効果的に低下させる.
- BPSCSKランチバイオティクスは,ニシンAと比較して,共生細菌に対する活性が低下しています.
- 患者およびマウスの高いランティバイオティクスの遺伝子は,VRE密度の低下と相関しています.
結論:
- BPSCSKのような抗生物質を生成する共生細菌は,腸内でのVREの植民を効果的に減らすことができます.
- BPSCSK由来のランチビオティックは,有益な腸内細菌に少ない影響を及ぼす標的型アプローチを提供します.
- これらの発見は,VRE感染と戦うためにプロバイオティクスとしてランチバイオティクスを生産するコメンサルの開発を支持する.
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