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Binary Fission01:26

Binary Fission

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Binary fission is the primary mode of asexual reproduction in prokaryotes, such as bacteria. It results in the production of two genetically identical daughter cells. This highly efficient process ensures the rapid propagation of bacterial populations under favorable conditions and involves coordinated cellular and molecular events.DNA Replication and SeparationThe process begins with the replication of the bacterial chromosome. The circular DNA molecule unwinds at a specific origin of...
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Binary Fission01:20

Binary Fission

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Fission is the division of a single entity into two or more parts, which regenerate into separate entities that resemble the original. Organisms in the Archaea and Bacteria domains reproduce using binary fission, in which a parent cell splits into two parts that can each grow to the size of the original parent cell. This asexual method of reproduction produces cells that are all genetically identical.
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lncRNA - Long Non-coding RNAs02:39

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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Lewis Acids and Bases02:33

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In 1923, G. N. Lewis proposed a generalized definition of acid-base behavior in which acids and bases are identified by their ability to accept or to donate a pair of electrons and form a coordinate covalent bond.
A coordinate covalent bond (or dative bond) occurs when one of the atoms in the bond provides both bonding electrons. For example, a coordinate covalent bond occurs when a water molecule combines with a hydrogen ion to form a hydronium ion. A coordinate covalent bond also results when...
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The Nursing Code of Ethics sets the ethical benchmark for the profession, and guides nurses in ethical analysis and decision making at the societal, organizational, and clinical levels. The code encompasses showing compassion and respect for the patient, their families, and communities in all circumstances while committing to providing patient-centered care. In addition, the code states that nurses must advocate for the patient by defending a cause or recommendation to protect their rights,...
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動的ボロン酸/カテコールバイナリコードによる配列プログラミング

Marco Hebel1,2, Andreas Riegger2, Maksymilian M Zegota1,2

  • 1Max Planck Institute for Polymer Research , Ackermannweg 10 , 55128 Mainz , Germany.

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|August 23, 2019
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まとめ

科学者はボロン酸とカテキールを用いて 合成コードを作成し DNAを模倣して 知的分子システムを作りました このシステムはシーケンス固有の認識と プログラム可能な脱ハイブリッド化を可能にし, 分子プログラミングを進める.

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科学分野:

  • 分子生物学
  • 合成化学
  • 生物化学

背景:

  • インテリジェントな分子システムを発展させるには DNAのようなプログラム可能な 合成システムを開発することが重要です
  • ダイナミックな共性化学は,新しい分子相互作用を生み出すための多用途のプラットフォームを提供します.

研究 の 目的:

  • 配列特異的な認識のためにボロン酸-カテコール相互作用を使用して合成核塩基類を設計する.
  • この合成システムの熱力学的な性質と動的鎖の位移とエラー率を調査する.
  • プログラム可能な分子組立のためのpH依存の脱ハイブリッド化とマクロ分子結合を実証する.

主な方法:

  • ボロン酸 (BA) とカテキール (CA) の残基をペプチド骨格に組み込む.
  • ダイナミックストランドの移動とハイブリッド化の熱力学分析
  • pHに依存する結合と脱ハイブリッド化運動の調査.
  • タンパク質を補完的な合成糸に結合させる.

主要な成果:

  • BA/CA相互作用によってシーケンス固有の結合を可能にする合成核塩基アナログ.
  • 熱力学的に制御された糸の移動と特定のパートナー選択が実証されています.
  • DNAと異なるpH5.0でpH依存の脱ハイブリッド化が達成された.
  • PEG鎖にシトクロームcタンパク質をうまく結合し,マクロ分子認識を示した.

結論:

  • 開発された合成コードは 配列プログラム可能な分子システムに 新しいプラットフォームを提供します
  • BA / CAの相互作用のpH調節性は,分子制御と脱ハイブリッド化にユニークな利点を提供します.
  • このアプローチは分子レベルとマクロ分子レベルの両方でシーケンスの認識を可能にし,高度な分子機械への道を切り開きます.