プログラム可能なCRISPR対応のスマート素材
Max A English1,2, Luis R Soenksen2,3,4, Raphael V Gayet1,2,5
1Department of Biological Engineering, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA.
Science (New York, N.Y.)
|August 24, 2019
まとめ
研究者はCRISPR-Cas12a技術を使って生物学的信号に反応するDNAベースの新型ヒドロゲルを開発しました. これらのスマート素材は化合物を放出したり 劣化させたり バイオテクノロジーのアプリケーションの 電気フィュージとして機能します
科学分野:
- バイオマテリアル科学
- 合成生物学
- バイオテクノロジー
背景:
- 刺激に反応する材料は 先進的なバイオテクノロジーにとって不可欠です
- CRISPRに関連したヌクレアスは 物質の動作をプログラムできます
研究 の 目的:
- CRISPR-Cas12aを使って 生物学的信号で活性化される DNAベースの水素ゲルを設計する
- これらのプログラム可能な生体材料を活用した様々な in vitro アプリケーションを実証する.
主な方法:
- CRISPR-Cas12aヌクレアスを利用して DNAをヒドロゲル構造で割ります
- 構造要素としてDNAを組み込むか,ヒドロゲルにペンダントグループを固定する.
- 枝分かれしたポリエチレングリコール,ポリアクリルアミド-DNA,炭素黒-DNAのヒドロゲルを開発する.
主要な成果:
- 4つの異なるアプリケーションを実証しました. 薬物放出,ナノ粒子/細胞封じ込め,分解可能な電気フィューズ,流体バルブ.
- 生物学的情報を 測定可能な物質特性に 変換した.
- ガイドRNAで定義されたインプットによる水素ゲルのプログラム性を示した.
結論:
- CRISPR-Cas12aで動作するDNAベースのヒドロゲルは バイオテクノロジーの汎用性のあるプラットフォームです
- これらの材料は,組織工学,バイオエレクトロニクス,診断における新しい in vitro アプリケーションを可能にします.
- 生物学的シグナルに対する物質の反応を正確に制御できる.
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