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関連する概念動画

Induced-fit Model01:13

Induced-fit Model

88.9K
Most chemical reactions in cells require enzymes—biological catalysts that speed up the reaction without being consumed or permanently changed. They reduce the activation energy needed to convert the reactants into products. Enzymes are proteins, that usually work by binding to a substrate—a reactant molecule that they act upon.
Enzymes exhibit substrate specificity, meaning that they can only bind to certain substrates. This is mainly determined by the shape and chemical...
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Goodness-of-Fit Test01:16

Goodness-of-Fit Test

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The goodness-of-fit test is a type of hypothesis test which determines whether the data "fits" a particular distribution. For example, one may suspect that some anonymous data may fit a binomial distribution. A chi-square test (meaning the distribution for the hypothesis test is chi-square) can be used to determine if there is a fit. The null and alternative hypotheses may be written in sentences or stated as equations or inequalities. The test statistic for a goodness-of-fit test is given as...
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Inclusive Fitness00:57

Inclusive Fitness

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Most altruistic behavior—in which one animal helps another at a cost to themselves—occurs between relatives. Scientists think these altruistic behaviors evolved because they increase the inclusive fitness of the animal providing help.
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Intermolecular Forces03:13

Intermolecular Forces

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Atoms and molecules interact through bonds (or forces): intramolecular and intermolecular. The forces are electrostatic as they arise from interactions (attractive or repulsive) between charged species (permanent, partial, or temporary charges) and exist with varying strengths between ions, polar, nonpolar, and neutral molecules. The different types of intermolecular forces are ion–dipole, dipole–dipole, hydrogen bonds, and dispersion; among these, dipole–dipole, hydrogen...
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Electromotive Force02:36

Electromotive Force

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Electricity is generated by either electrons or ions flowing through a solution or a conducting medium. This flow of electrons or specifically electrical charge is defined as an electric current. When electrons move through a wire, they generate an electric current. It can be recalled  that in a redox reaction, electrons are lost and gained. In the spontaneous redox reaction of zinc  with copper, when zinc is immersed in a copper ion solution, a transfer of electrons from one substance to...
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Expected Frequencies in Goodness-of-Fit Tests01:19

Expected Frequencies in Goodness-of-Fit Tests

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A goodness-of-fit test is conducted to determine whether the observed frequency values are statistically similar to the frequencies expected for the dataset. Suppose the expected frequencies for a dataset are equal such as when predicting the frequency of any number appearing when casting a die. In that case, the expected frequency is the ratio of the total number of observations (n)  to the number of categories (k).
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Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
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強制インターケレーション (FIT) - アプタマー

Sasha B Ebrahimi, Devleena Samanta, Ho Fung Cheng

    Journal of the American Chemical Society
    |August 24, 2019
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    まとめ
    この要約は機械生成です。

    研究者は新しいシグナリングアプタマークラスである強制インターケレーション (FIT) アプタマーを開発した. これらのアプタマーは,複雑な生物サンプルにおける様々な標的の敏感で特異的な光検出を可能にする単一の修正を使用します.

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    科学分野:

    • 生物化学
    • 分子生物学
    • 分析化学

    背景:

    • アプタマーは,分析物検出のための多用途のオリゴヌクレオチドツールです.
    • 既存のアプタマーシグナリング方法はしばしば偽陽性または限られたダイナミックレンジに苦しんでいます.

    研究 の 目的:

    • 頑丈な光読み取りのための一般的なアプタマー設計戦略を開発する.
    • 性能が向上した新種のシグナリングアプタマーを作成する.

    主な方法:

    • アプタマー・ターゲット・コンプレックスに 浸透する粘度感受性染料を用いた
    • 標的結合時に光性を増加させる強制インターケレーション (FIT) アプタマーを開発した.
    • 構造変化,サンドイッチアッセイ,スプリットアプタマー再結合を含む様々な結合モードでFITアプタマーの有用性を実証した.

    主要な成果:

    • FITアプタマーは,標的結合時に光を20倍まで増加させました.
    • 戦略はメタロベースペア形成を成功裏に検出しました.
    • FITアプタマーは従来のシステムと比較して偽陽性信号の減少を示した.
    • ヒトの血清で高い信号対背景比 (FRETより最大15倍) とナノモラー感度を達成した.

    結論:

    • FITアプタマーは,単一の修正を伴う新しいシグナリングアプタマーのクラスです.
    • この戦略により,複雑なメディアにおける多様な標的の 敏感で具体的な検出が可能になります.
    • FITアプタマーは,既存のアプタマーベースの検出方法の有望な代替手段です.