水素-デウテリウム交換質量スペクトロメトリを用いたランチペプチド合成酵素の動的構造特性に関する洞察
Yeganeh Habibi1, Kevin A Uggowitzer1, Hassan Issak1
1McGill University , Department of Chemistry , 801 Sherbrooke Street , West Montréal , Québec , Canada H3A 0B8.
Journal of the American Chemical Society
|August 27, 2019
まとめ
この研究では,リボソーム合成および翻訳後に改変されたペプチド (RiPPs) のバイオシンセシスに不可欠なHalM2酵素の動的構造を明らかにするために,水素-デュテリウム交換質量スペクトロメトリ (HDX-MS) を使用しています.
科学分野:
- 生物化学
- 構造生物学
- 分子生物学
背景:
- リボソーム合成および翻訳後の改変ペプチド (RiPPs) は,複雑な酵素経路によって生物合成される.
- 酵素構成のダイナミクスは,RiPPの改変結果に影響を与える可能性があるが,直接的な証拠は限られている.
- これらのダイナミクスを特徴づけることは,RiPPの生物合成と工学を理解するための鍵です.
研究 の 目的:
- RiPP生物合成酵素の生体物理的性質を調査するために,水素-デウテリウム交換質量スペクトロメトリ (HDX-MS) を包括的に適用する.
- クラスIIランチペプチド合成酵素HalM2の構造的動態を解明する.
- HalM2-HalA2前駆ペプチドシステム内の新しい相互作用と機能的要素を特定する.
主な方法:
- 主要な技術として水素-デュテリウム交換質量スペクトロメトリ (HDX-MS) を利用した.
- モデルシステムとしてクラスIIランチペプチド合成酵素HalM2を使用した.
- HDX-MSデータによる変異酵素の生化学分析と運動分析を行った.
主要な成果:
- ハルM2が構造的にダイナミックな酵素であることを示した.
- ハルM2内の新しい前駆ペプチド結合要素を特定した.
- リガンド結合とATP水解によって誘発されたHalM2の長距離構造的通信を発見した.
- HalM2とHalA2の前駆ペプチドサブドメインの間の特定の相互作用が検出されました.
結論:
- HDX-MSは,HalM2のようなRiPP生物合成酵素の構造ダイナミクスに関する基本的な洞察を提供します.
- 識別された動的要素と相互作用は,機能的に関連しており,生化学的測定で検証することができます.
- この研究は,LanMシステムの理解を深め,RiPPの生物合成の合理的な工学を可能にします.
関連する概念動画
Tandem Mass Spectrometry
2.4K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
2.4K
Mass Spectrometry: Overview
8.3K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
8.3K
Mass Spectrometry of Amines
5.3K
In mass spectroscopy, amines undergo fragmentation to give parent ions with odd molecule weights. This observed mass spectrum follows the nitrogen rule; a molecule with an odd number of nitrogen atoms produces a molecular ion with an odd molecular weight. Amines undergo fragmentation through α cleavage, producing nitrogen-containing cations—iminium ions—and alkyl radicals. Mass spectra of aromatic and cyclic aliphatic amines exhibit strong molecular ion peaks, but acyclic...
5.3K
Mass Spectrometry: Isotope Effect
3.9K
Most elements exist in nature as a mixture of isotopes. The isotopes differ in weight due to their respective number of neutrons. The molecular weight of a molecule is different depending on the specific isotope of its elements involved. As a result, the mass spectrum of the molecule exhibits peaks from the same fragment at multiple positions. The positions of these mass signals depend on the mass differences between isotopes. Furthermore, the intensity of these signals is dependent on the...
3.9K
Structural Properties and Dimensions of Lumber
383
Wood's structural properties derive from fibers aligned along the tree's length, contributing significantly to its mechanical strength. Wood exhibits up to twenty times greater tensile strength along these fibers compared to across them, and generally shows better performance under compression than tension. The length of fibers varies, with hardwoods having fibers around one twenty-fifth inch long and softwoods ranging from one-eighth to one-third inch.
The strength characteristics of...
The strength characteristics of...
383
Structure and Physical Properties of Alkynes
13.1K
Introduction:
In nature, compounds containing both carbon and hydrogen are known as "hydrocarbons". Aliphatic hydrocarbons are compounds whose molecules contain saturated single bonds (i.e., alkanes) or unsaturated double or triple bonds. Alkenes contain carbon–carbon double bonds and have a structural formula CnH2n. Unsaturated hydrocarbons containing carbon–carbon triple bonds are called "alkynes" and are structurally represented by the formula CnH2n-2.
The...
In nature, compounds containing both carbon and hydrogen are known as "hydrocarbons". Aliphatic hydrocarbons are compounds whose molecules contain saturated single bonds (i.e., alkanes) or unsaturated double or triple bonds. Alkenes contain carbon–carbon double bonds and have a structural formula CnH2n. Unsaturated hydrocarbons containing carbon–carbon triple bonds are called "alkynes" and are structurally represented by the formula CnH2n-2.
The...
13.1K


