関連する実験動画

Updated: Jan 20, 2026

Preparation, Purification, and Use of Fatty Acid-containing Liposomes
10:43

Preparation, Purification, and Use of Fatty Acid-containing Liposomes

Published on: February 9, 2018

82.2K

脂肪酸とがん増幅されたZDHHC19は,S-パルミトイレーションを通じてSTAT3活性化を促進する

Jixiao Niu1, Yang Sun1, Baoen Chen1

  • 1Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, USA.

Nature
|August 30, 2019
PubMed
まとめ

シグナルトランスデューサーとトランスクリプション3のアクティベーター (STAT3) は,脂肪酸とZDHHC19によって調節されるS-palmitoylationによって活性化されます. この発見は,パルミトイレーションの緩和と炎症と癌を関連付けています.

さらに関連する動画

Identification of Fatty Acids in Bacillus cereus08:41

Identification of Fatty Acids in Bacillus cereus

Published on: December 5, 2016

10.1K
Analysis of Fatty Acid Content and Composition in Microalgae07:44

Analysis of Fatty Acid Content and Composition in Microalgae

Published on: October 1, 2013

61.5K

関連する概念動画

Preparation, Purification, and Use of Fatty Acid-containing Liposomes10:43

Preparation, Purification, and Use of Fatty Acid-containing Liposomes

Liposomes containing single-chain amphiphiles, particularly fatty acids, exhibit distinct properties compared to those containing diacylphospholipids due to the unique chemical properties of single chain amphiphiles. Here we describe techniques for the preparation, purification, and use of liposomes comprised in part or whole of these...
82.2K
Identification of Fatty Acids in Bacillus cereus08:41

Identification of Fatty Acids in Bacillus cereus

We propose a protocol to identify fatty acids without the need to purify them. It combines information on the retention times with the mass spectra of three types of fatty acid derivatives: fatty acid methyl esters (FAMEs), 4,4-dimethyl oxazoline derivatives (DMOX), and 3-pyridylcarbinyl esters (picolinyl).
10.1K
Analysis of Fatty Acid Content and Composition in Microalgae07:44

Analysis of Fatty Acid Content and Composition in Microalgae

A method for the determination of fatty acid content and composition in microalgae based on mechanical cell disruption, solvent based lipid extraction, transesterification, and quantification and identification of fatty acids using gas chromatography is described. A tripentadecanoin internal standard is used to compensate for the possible losses during extraction and incomplete...
61.5K
Saturated Fatty Acids Induce Ceramide-associated Macrophage Cell Death08:26

Saturated Fatty Acids Induce Ceramide-associated Macrophage Cell Death

We illustrate a straight-forward method to derive murine primary macrophages from bone marrow cells and a simple method to prepare BSA-fatty acid conjugates. Then we demonstrate that saturated fatty acids can induce macrophage cell death, and such cell death is positively associated with cellular accumulation of ceramide...
9.7K
Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry07:27

Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry

The study of fatty acylation has important implications in cellular protein interactions and diseases. Presented here is a modified protocol to improve click chemistry detection of fatty acylated proteins, which can be applied in various cell types and combined with other assays, including pulse-chase and mass...
6.0K
Determination of Fatty Acid Oxidation and Lipogenesis in Mouse Primary Hepatocytes12:11

Determination of Fatty Acid Oxidation and Lipogenesis in Mouse Primary Hepatocytes

De novo lipogenesis and β-fatty acid oxidation constitute key metabolic pathways in hepatocyte, pathways that are perturbed in several metabolic disorders, including fatty liver disease. Here we demonstrate isolation of mouse primary hepatocytes and describe quantification of β-fatty acid oxidation and...
20.8K