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Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores09:43

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A methodology for preparing solid-state nanopores in solution for biomolecular translocation experiments is presented. By applying short pulses of high electric fields, the nanopore diameter can be controllably enlarged with subnanometer precision and its electrical noise characteristics significantly improved. This procedure is performed in situ using standard laboratory equipment under experimental...
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This protocol presents a set of experiments adopted for the evaluation of photoswitchable anticancer peptides, that can be used in the preclinical screening of such compounds. This includes cytotoxicity assessment in 2D and 3D cell cultures, the evaluation of ex vivo (model tissue) photoisomerization efficiency, and in vivo...
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We present a protocol to build molecular shuttles, where surface-adhered kinesin motor proteins propel dye-labelled microtubules. Weak interactions of the kinesins with the surface enables their reversible attachment to it. This creates a nanoscale system which exhibits dynamic assembly and disassembly of its components while retaining its...
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Nanopore sequencing is a novel technology that allows cost-effective sequencing in remote locations and resource-poor settings. Here, we present a protocol for sequencing of mRNAs from whole blood that is compatible with such...
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We previously validated a protocol for amplicon-based whole genome Usutu virus (USUV) sequencing on a nanopore sequencing platform. Here, we describe the methods used in more detail and determine the error rate of the nanopore R10 flow...
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Nanoporous gold with a hierarchical and bimodal pore size distribution can be produced by combining electrochemical and chemical dealloying. The composition of the alloy can be monitored via EDS-SEM examination as the dealloying process advances. The material's loading capacity can be determined by studying protein adsorption onto the...
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関連する実験動画

Updated: Jan 20, 2026

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores
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リバーシブル・フォト制御ナノポーア・アセンブリ

Natalie L Mutter, Jana Volarić, Wiktor Szymanski1

  • 1University Medical Center Groningen, Department of Radiology , University of Groningen , Hanzeplein 1 , 9713 GZ , Groningen , The Netherlands.

Journal of the American Chemical Society
|August 31, 2019
PubMed
まとめ

科学者たちは 細胞膜の孔形成を 光で制御するために フレガセアトキシンC (FraC) を設計しました この光活性化毒素は 標的型がん治療と 先進的なナノ孔センサー技術に 潜在力を示しています

科学分野:

  • 生物化学
  • バイオ物理学
  • 材料科学

背景:

  • セルラー機能を調節する方法を提供します セルラー機能を調節する方法を提供します セルラー機能を調節する方法を提供します
  • フラガセアトキシンC (FraC) は細胞膜ナノ孔を形成し,細胞死を引き起こす.
  • FraCの自己組み立てを制御することで 細胞のプロセスを正確に制御できます

研究 の 目的:

  • ナノ孔組成の遠隔制御のための光制御フラガセアトキシンC (FraC) を開発する.
  • 癌治療薬とナノ孔センサー装置における光制御FraCの可能性を調査する.

主な方法:

  • フォトスイッチ可能なアボベンゼンペンダントは,スフィンゴミエリン結合ポケットの近くにFraCに接続されました.
  • 修正されたFraCの結合親和性と毛穴形成活性は,光照射によって評価された.
  • 細胞解離試験と人工脂質膜実験が,光制御を評価するために実施された.

主要な成果:

  • 改造されたFraC構造は,細胞膜に可逆的な光活性化または無活性化結合を示した.
  • 熱状態で不活性な1つのFraCコンストラクタは,光にさらされると完全な癌細胞溶解を誘導した.
  • 選択的光照射により,人工の脂質膜に個々のナノ孔を分離することが可能になった.

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関連する実験動画

Last Updated: Jan 20, 2026

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores
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Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores

Published on: October 31, 2013

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Published on: January 26, 2019

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結論:

  • 光制御FraCはナノ孔形成の精密な時空調節のための新しい方法を提供します.
  • この技術は,光薬学,特にがん治療の応用が期待されています.
  • フォト制御FraCは,高度なセンシングデバイスでナノ孔配列を製造するために利用できます.