関連する実験動画

Updated: Jan 20, 2026

Author Spotlight: Membrane Protein Reconstitution in Synthetic Cells
07:14

Author Spotlight: Membrane Protein Reconstitution in Synthetic Cells

Published on: March 8, 2024

1.7K

グルタマート 受容体 遺伝子 に よっ て 暗号化 さ れ た 寒さ を 感知 する 受容体

Jianke Gong1, Jinzhi Liu1, Elizabeth A Ronan2

  • 1College of Life Science and Technology, Key Laboratory of Molecular Biophysics of MOE, and International Research Center for Sensory Biology and Technology of MOST, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China; Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA; Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, MI 48109, USA.

Cell
|September 3, 2019
PubMed
まとめ

科学者は,グルタミン酸受容体である GLR-3 遺伝子が,外周神経系における 寒さセンサーとして作用することを発見しました. この発見は 低温を検出するための 進化的に保存されたメカニズムを示しています

さらに関連する動画

An In Vitro Technique to Study Glutamate Receptor Trafficking in Hippocampal Neurons05:27

An In Vitro Technique to Study Glutamate Receptor Trafficking in Hippocampal Neurons

496
A High-throughput Calcium-flux Assay to Study NMDA-receptors with Sensitivity to Glycine/D-serine and Glutamate04:48

A High-throughput Calcium-flux Assay to Study NMDA-receptors with Sensitivity to Glycine/D-serine and Glutamate

Published on: July 10, 2018

9.7K

関連する概念動画

Reconstitution of the Bacterial Glutamate Receptor Channel by Encapsulation of a Cell-Free Expression System07:14

Reconstitution of the Bacterial Glutamate Receptor Channel by Encapsulation of a Cell-Free Expression System

This protocol describes the inverted emulsion method used to encapsulate a cell-free expression (CFE) system within a giant unilamellar vesicle (GUV) for the investigation of the synthesis and incorporation of a model membrane protein into the lipid...
1.7K
An In Vitro Technique to Study Glutamate Receptor Trafficking in Hippocampal Neurons05:27

An In Vitro Technique to Study Glutamate Receptor Trafficking in Hippocampal Neurons

This video demonstrates an antibody-feeding approach to study the trafficking of glutamate receptors in primary hippocampal neuronal cultures. This approach allows the observation of receptor populations at both the plasma and internal membranes. Discrimination between these subsets is achieved by labeling the receptors before and after membrane permeabilization, using the same primary antibody but secondary antibodies conjugated to different...
496
A High-throughput Calcium-flux Assay to Study NMDA-receptors with Sensitivity to Glycine/D-serine and Glutamate04:48

A High-throughput Calcium-flux Assay to Study NMDA-receptors with Sensitivity to Glycine/D-serine and Glutamate

The goal of this protocol is to facilitate the study of NMDA-receptors (NMDAR) at a larger scale and allow the examination of modulatory effects of small molecules and their therapeutic...
9.7K
Internal Receptors01:31

Internal Receptors

Many cellular signals are hydrophilic and therefore cannot pass through the plasma membrane. However, small or hydrophobic signaling molecules can cross the hydrophobic core of the plasma membrane and bind to internal, or intracellular, receptors that reside within the cell. Many mammalian steroid hormones use this mechanism of cell signaling, as does nitric oxide (NO) gas.
74.3K
An Antibody Feeding Approach to Study Glutamate Receptor Trafficking in Dissociated Primary Hippocampal Cultures08:13

An Antibody Feeding Approach to Study Glutamate Receptor Trafficking in Dissociated Primary Hippocampal Cultures

This article presents a method to study glutamate receptor (GluR) trafficking in dissociated primary hippocampal cultures. Using an antibody-feeding approach to label endogenous or overexpressed receptors in combination with pharmacological approaches, this method allows for the identification of molecular mechanisms regulating GluR surface expression by modulating internalization or recycling...
6.1K
Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors16:16

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

We genetically-encode the unnatural amino acid, p-azido-L-phenylalanine at various targeted positions in GPCRs and show the versatility of the azido group in different applications. These include a targeted photocrosslinking technology to identify residues in the ligand-binding pocket of a GPCR, and site-specific bioorthogonal modification of GPCRs with a peptide-epitope tag or fluorescent...
15.7K