関連する実験動画

Updated: Jan 20, 2026

CRISPR-Mediated Base Editing Tools: A Genome Editing Technique to Induce Targeted Base Substitution
02:58

CRISPR-Mediated Base Editing Tools: A Genome Editing Technique to Induce Targeted Base Substitution

4.0K

CRISPRによるゲノム編集と転写のライブイメージング

Haifeng Wang1, Muneaki Nakamura1, Timothy R Abbott1

  • 1Department of Bioengineering, Stanford University, Stanford, CA 94305, USA.

Science (New York, N.Y.)
|September 7, 2019
PubMed
まとめ

CRISPR LiveFISHを開発しました 生きた細胞のゲノムをリアルタイムで追跡する 新しい方法です ゲノム編集や染色体再編の過程で DNAとRNAの動態を視覚化します

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CRISPR-Mediated Base Editing Tools: A Genome Editing Technique to Induce Targeted Base Substitution02:58

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This video explains the concept of CRISPR-mediated cytosine base editors for inducing targeted nucleotide...
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A well-established technique for modifying specific sequences in the genome is gene targeting by homologous recombination, but this method can be laborious and only works in certain organisms. Recent advances have led to the development of “genome editing”, which works by inducing double-strand breaks in DNA using engineered nuclease enzymes guided to target genomic sites by either proteins or RNAs that recognize specific sequences. When a cell attempts to repair this damage,...
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Efficient genome engineering of Candida albicans is critical to understanding the pathogenesis and development of therapeutics. Here, we described a protocol to quickly and accurately edit the C. albicans genome using CRISPR. The protocol allows investigators to introduce a wide variety of genetic modifications including point mutations, insertions, and deletions.
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CRISPR-Cas is a powerful technology to engineer the complex genomes of plants and animals. Here, we detail a protocol to efficiently edit the human genome using different Cas endonucleases. We highlight important considerations and design parameters to optimize editing...
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This protocol describes an approach to facilitate precise knock-in edits in zebrafish embryos using CRISPR-Cas9 technology. A phenotyping pipeline is presented to demonstrate the applicability of these techniques to model a Long QT Syndrome-associated gene...
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Here we provide a detailed protocol to carry out in vivo cardiac gene editing in mice using recombinant Adeno-Associated Virus(rAAV)-mediated delivery of CRISPR. This protocol offers a promising therapeutic strategy to treat dystrophic cardiomyopathy in Duchenne muscular dystrophy and can be used to generate cardiac-specific knockout in postnatal...
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