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Updated: Jun 15, 2026

Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers
10:08

Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers

Published on: July 25, 2012

時間の解像度3D単分子追跡法を使用して,生細胞におけるDNA混合動力学的測定

Yuan-I Chen1, Yin-Jui Chang2, Trung Duc Nguyen1

  • 1Department of Biomedical Engineering , University of Texas at Austin , Austin , Texas 78712 , United States.

Journal of the American Chemical Society
|September 12, 2019
PubMed
まとめ

研究者は3D追跡方法を開発し 細胞内でDNAの解熱と溶解を観察しました これは,インビトロ条件と比較して,細胞環境でのDNAハイブリッド化が著しく速いことを明らかにした.

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Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking

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Mapping Absolute DNA Density in Cell Nuclei using Single-molecule Localization Microscopy10:57

Mapping Absolute DNA Density in Cell Nuclei using Single-molecule Localization Microscopy

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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