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Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro10:01

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro

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At the interface of organic and aqueous solvents, tailored amphiphilic elastin-like proteins assemble into complex supramolecular structures such as vesicles, fibers and coacervates triggered by environmental parameters. The described assembly protocols yield Protein Membrane-Based Compartments (PMBCs) with tunable properties, enabling the encapsulation of various...
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Synthesis and Characterization of Supramolecular Colloids09:26

Synthesis and Characterization of Supramolecular Colloids

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A protocol for the synthesis and characterization of colloids coated with supramolecular moieties is described. These supramolecular colloids undergo self-assembly upon the activation of the hydrogen-bonds between the surface-anchored molecules by...
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A Uniform Shear Assay for Human Platelet and Cell Surface Receptors via Cone-plate Viscometry04:32

A Uniform Shear Assay for Human Platelet and Cell Surface Receptors via Cone-plate Viscometry

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We describe an in-solution method to apply uniform shear to platelet surface receptors using cone-plate viscometry. This method may also be used more broadly to apply shear to other cell types and cell-fragments and need not target a specific ligand-receptor...
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Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets05:49

Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets

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Procoagulant platelet formation has been correlated with an increased risk of thrombosis. Presented here is a precise protocol for isolating washed platelets from human blood, intended to quantify the exposure of phosphatidylserine and microvesicle release, which are distinctive features of procoagulant...
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Studying the Supramolecular Organization of Photosynthetic Membranes within Freeze-fractured Leaf Tissues by Cryo-scanning Electron Microscopy13:52

Studying the Supramolecular Organization of Photosynthetic Membranes within Freeze-fractured Leaf Tissues by Cryo-scanning Electron Microscopy

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Here we describe a procedure for studying freeze-fractured plant tissues. High-pressure frozen leaf samples are freeze-fractured and double-layer coated, yielding well preserved frozen-hydrated samples that are imaged using the cryo-scanning electron microscope at high magnifications with minimal beam...
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Uniform Distribution01:19

Uniform Distribution

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The uniform distribution is a continuous probability distribution of events with an equal probability of occurrence. This distribution is rectangular.
Two essential properties of this distribution are
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関連する実験動画

Updated: Jan 19, 2026

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro
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均一 で 精確 に 制御 さ れ て いる 超 分子 六角 型 血小板 組

Gangfeng Chen, Guangpu Zhang, Bixin Jin

  • 1RIKEN Centre for Emergent Matter Science , Saitama 351-0198 , Japan.

Journal of the American Chemical Society
|September 12, 2019
PubMed
まとめ

研究者らは,結晶インクルージョン複合体を用いて,柔らかい材料から正確な六角形の血小板を作成する新しい方法を開発しました. この超分子組立技術は 制御された寸法と同心六角構造の形成を可能にします

さらに関連する動画

Synthesis and Characterization of Supramolecular Colloids
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A Uniform Shear Assay for Human Platelet and Cell Surface Receptors via Cone-plate Viscometry
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A Uniform Shear Assay for Human Platelet and Cell Surface Receptors via Cone-plate Viscometry

Published on: June 5, 2019

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関連する実験動画

Last Updated: Jan 19, 2026

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro
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Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro

Published on: April 8, 2020

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Synthesis and Characterization of Supramolecular Colloids
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Synthesis and Characterization of Supramolecular Colloids

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A Uniform Shear Assay for Human Platelet and Cell Surface Receptors via Cone-plate Viscometry
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科学分野:

  • 材料科学
  • 超分子化学
  • ナノテクノロジー

背景:

  • 幾何学的な構造は 自然と工学的なシステムに 広く見られます
  • 特に柔らかい素材から 精密な幾何学的な組み立てを ボトムアップで作り出すことは 依然として大きな課題です

研究 の 目的:

  • コロイド的に安定した六角形の血小板を製造するための一般的な方法を示します.
  • 柔らかい材料から幾何学的な組み立ての合成で精密な寸法制御を達成する.

主な方法:

  • Tris-o-phenylenedioxycyclotriphosphazeneとブロックコポリマーを使用して結晶インクルージョン複合体の形成.
  • 血小板形成のためのインクルージョン複合体内のスクリュー脱位成長を利用する.
  • 同心六角形の血小板を作るための段階的な種子成長を使用します.

主要な成果:

  • 制御可能な寸法を持つ均一な六角形の血小板を成功裏に製造した.
  • 同心六角形の血小板を 種で育てる方法によって 生成する能力を示した.
  • この方法により,コロイド的に安定した六角形の組成物が得られます.

結論:

  • 六角形の血小板を製造するための一般的で効果的な超分子組立法が確立されています.
  • 精密な寸法制御は,結晶インクルージョン複合体におけるスクルー脱位成長によって達成可能である.
  • このアプローチは 柔らかい素材から複雑な幾何学的な構造を作り出すための 経路を提供します