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関連する概念動画

Electron Transport Chains01:28

Electron Transport Chains

111.6K
The final stage of cellular respiration is oxidative phosphorylation that consists of two steps: the electron transport chain and chemiosmosis. The electron transport chain is a set of proteins found in the inner mitochondrial membrane in eukaryotic cells. Its primary function is to establish a proton gradient that can be used during chemiosmosis to produce ATP and generate electron carriers, such as NAD+ and FAD, that are used in glycolysis and the citric acid cycle.
The ETC is comprised of...
111.6K
Ultra-High-Speed Western Blot using Immunoreaction Enhancing Technology05:59

Ultra-High-Speed Western Blot using Immunoreaction Enhancing Technology

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An ultra-high-speed western blotting technique is developed by improving the kinetics of antigen-antibody binding through cyclic draining and replenishing (CDR) technology in conjunction with an immunoreaction enhancing...
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Cyclic Voltammetry (CV)08:37

Cyclic Voltammetry (CV)

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Source: Laboratory of Dr. Kayla Green — Texas Christian University
A Cyclic Voltammetry (CV) experiment involves the scan of a range of potential voltages while measuring current. In the CV experiment, the potential of an immersed, stationary electrode is scanned from a predetermined starting potential to a final value (called the switching potential) and then the reverse scan is obtained. This gives a 'cyclic' sweep of potentials and the current vs. potential curve derived from...
129.7K
Facilitated Transport01:19

Facilitated Transport

146.3K
The chemical and physical properties of plasma membranes cause them to be selectively permeable. Since plasma membranes have both hydrophobic and hydrophilic regions, substances need to be able to transverse both regions. The hydrophobic area of membranes repels substances such as charged ions. Therefore, such substances need special membrane proteins to cross a membrane successfully. In  facilitated transport, also known as facilitated diffusion, molecules and ions travel across a...
146.3K
Stereoisomerism of Cyclic Compounds02:33

Stereoisomerism of Cyclic Compounds

10.9K
In this lesson, we delve into the role of ring conformation and its stability, which determines the spatial arrangement and, consequently, the molecular symmetry and stereoisomerism of cyclic compounds. 1,2-Dimethylcyclohexane is used as a case study to evaluate the possible number of stereoisomers. Here, given the multiple (n = 2) chiral centers, there are 2n = 4 possible configurations that lack a plane of symmetry, as the ring skeleton exists in a non-planar chair conformation. In addition,...
10.9K
Primary Active Transport01:47

Primary Active Transport

196.6K
In contrast to passive transport, active transport involves a substance being moved through membranes in a direction against its concentration or electrochemical gradient. There are two types of active transport: primary active transport and secondary active transport. Primary active transport utilizes chemical energy from ATP to drive protein pumps that are embedded in the cell membrane. With energy from ATP, the pumps transport ions against their electrochemical gradients—a direction...
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Ultra-High-Speed Western Blot using Immunoreaction Enhancing Technology
05:59

Ultra-High-Speed Western Blot using Immunoreaction Enhancing Technology

Published on: September 26, 2020

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SLC19A1は,免疫反応性周期性ダイヌクレオチドを輸送する.

Rutger D Luteijn1, Shivam A Zaver2, Benjamin G Gowen3,4

  • 1Department of Molecular and Cell Biology, and Cancer Research Laboratory, Division of Immunology and Pathogenesis, University of California, Berkeley, CA, USA.

Nature
|September 13, 2019
PubMed
まとめ
この要約は機械生成です。

研究者らは,細胞内に循環性ダイヌクレオチド (CDN) の主要なトランスポーターとして,減少葉酸キャリアSLC19A1を特定した. この発見は免疫反応や 癌の免疫療法や 炎症性疾患の理解に影響を与えます

さらに関連する動画

Cyclic Voltammetry CV: Measuring Redox Potentials and Currents
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Cyclic Voltammetry CV: Measuring Redox Potentials and Currents

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Electron Transport Chain, Ubiquinone and Cytochrome C
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Electron Transport Chain, Ubiquinone and Cytochrome C

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関連する実験動画

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Ultra-High-Speed Western Blot using Immunoreaction Enhancing Technology
05:59

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Published on: September 26, 2020

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Cyclic Voltammetry CV: Measuring Redox Potentials and Currents
08:37

Cyclic Voltammetry CV: Measuring Redox Potentials and Currents

Published on: April 30, 2023

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Electron Transport Chain, Ubiquinone and Cytochrome C
01:28

Electron Transport Chain, Ubiquinone and Cytochrome C

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科学分野:

  • 免疫学
  • 分子生物学
  • 細胞生物学

背景:

  • 細胞塩基DNAの蓄積は 感染,がん,DNA損傷の信号で cGAS-STING経路を活性化します
  • DNAによるcGASの活性化により,第2のメッセンジャーであるサイクルグアノシンモノフォスファート-アデノシンモノフォスファートが合成される.
  • 2' 3'- cGAMPはSTINGを活性化し,IRF3とNF- kBの活性化とI型インターフェロンの生成につながります.

研究 の 目的:

  • 周期性ダイヌクレオチド (CDN) が細胞膜を横断するメカニズムを特定する.
  • 標的細胞におけるSTING経路の活性化に不可欠な外来CDNの輸送を理解する.

主な方法:

  • ゲノム全体のCRISPR干渉スクリーンで,CDNトランスポーターを特定します.
  • 人間の細胞における候補トランスポーターの減少と過剰発現.
  • 各種の細胞のCDN吸収と機能的反応の評価

主要な成果:

  • 還元された葉酸媒介体SLC19A1は,CDNの主な媒介体として特定されました.
  • SLC19A1の枯渇はCDNの吸収と機能的反応を阻害した.
  • SLC19A1の過剰発現はCDNの吸収と反応を高めました.
  • 葉酸,スルファサラジン,メトトレクサートは,ヒト細胞におけるCDN吸収を抑制した.

結論:

  • SLC19A1は,CDNの細胞吸収を担う主要なトランスポーターです.
  • この発見は,がんの免疫療法,病原体に対する宿主反応,および炎症性疾患に重大な影響を及ぼします.