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Updated: Jan 19, 2026

Rapid Isolation of the Mitoribosome from HEK Cells
09:33

Rapid Isolation of the Mitoribosome from HEK Cells

Published on: October 4, 2018

11.7K

トリパノソームにおけるミトリボソーム小分体の生化には,広範な組み立て機構が含まれています

Martin Saurer1, David J F Ramrath1, Moritz Niemann2

  • 1Department of Biology, Institute of Molecular Biology and Biophysics, ETH Zurich, Otto-Stern-Weg 5, CH-8093 Zurich, Switzerland.

Science (New York, N.Y.)
|September 14, 2019
PubMed
まとめ

トリパノソーマ・ブルセイの小さなミトコンドリアのリボソームサブユニットの組み立てには,多数の要因が必要であり,中間複合体が含まれています. ミトコンドリアリボソームの生体生成に関する洞察を明らかにした4メガダルトンのアセンブルソーム中間体が見つかりました.

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Rapid Isolation of the Mitoribosome from HEK Cells09:33

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Mitochondria have specialized ribosomes that diverged from their bacterial and cytoplasmic counterparts. Here we show how mitoribosomes can be obtained from their native compartment in HEK cells. The method involves isolation of mitochondria from suspension cells and consequent purification of...
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This method of trypanosome separation from blood depends on their surface charge being less negative than mammalian blood cells. Infected blood is placed and treated on an anion-exchanger column. This method, the most fitting diagnostic for African trypanosomiasis, provides purified parasites for immunological, biological, biochemical, pharmaceutical and molecular biology...
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