関連する実験動画
Updated: Jan 19, 2026
04:46
Isolation of Lymphocytes from Mouse Genital Tract Mucosa
Published on: September 3, 2012
14.9K
まとめ
B細胞とT細胞の発見は 免疫学に革命をもたらしました マックス・クーパーとジャック・ミラー
科学分野:
- 免疫学と生物学の科学
- 癌や神経疾患などの疾患における免疫細胞の種類,起源,役割の探求
背景:
- 免疫学は急速に進歩する学際的な分野です.
- 免疫細胞の種類と起源の理解は 数十年前まで限られていた.
- 2019年のラスカー賞は,免疫学の基礎的な発見を称えました.
研究 の 目的:
- 免疫学の歴史的発展を強調する
- B細胞とT細胞の発見についてです
- 免疫学の研究の未来に 洞察を提供するためです
主な方法:
- 免疫学者のマックス・D・クーパーとの会話に基づいています
- 初期の免疫学の研究を論じたインタビューの抜粋を参考にしています
- その 会話 の 内容 を 関連 の 記事 で 読む こと が でき ます.
主要な成果:
- マックス・D・クーパーとジャック・ミラーはB細胞とT細胞を発見した.
- これらの発見は 現代の免疫学の基礎を築きました
- 新しい免疫細胞の種類と機能が 絶えず特定されています
結論:
- リンパ球の発見は 免疫学における重要な出来事でした
- 進行中の研究により 免疫系の複雑さに対する理解が 広がり続けています
- この分野は 医学や生物学における 将来の突破を約束しています
関連する概念動画
04:46Isolation of Lymphocytes from Mouse Genital Tract Mucosa
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An efficient way to isolate lymphocytes from mouse genital tract is described. This method takes advantage of enzyme digestion and Percoll gradient separation to allow efficient isolation. This technique is also adaptable to for use in other...
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08:08Isolation and Activation of Murine Lymphocytes
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Lymphocytes are the major players in adaptive immune responses. Here, we present a lymphocyte purification protocol to determine the physiological functions of the desired molecules in lymphocyte activation in vitro and in vivo. The described experimental procedures are suitable for comparing functional capacities between control and genetically modified...
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06:02Isolation of Lymphocytes from a Mouse Peyer's Patch
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In this video, we demonstrate the isolation of lymphocytes from a harvested mouse Peyer's patch. The isolated lymphocytes can be used for further...
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10:25Assessment of Lymphocyte Migration in an Ex Vivo Transmigration System
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In this protocol, lymphocytes are placed in the top chamber of a transmigration system, separated from the bottom chamber by a porous membrane. Chemokine is added to the bottom chamber, which induces active migration along a chemokine gradient. After 48 h, lymphocytes are counted in both chambers to quantitate...
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03:23An In Vitro Technique to Stimulate Lymphocytes via Pathogenic Bacteria
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This video demonstrates an in vitro technique to stimulate lymphocytes via non-typeable Haemophilus influenzae (NTHi) and to assess the immune response. Incubating isolated peripheral blood mononuclear cells (PBMCs) with NTHi causes the bacterial cells to stimulate T lymphocytes, leading to cytokine production. Upon inhibiting the secretion of cytokines, immunofluorescence staining is performed to detect the accumulated intracellular cytokines in stimulated T...
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11:28Isolating Lymphocytes from the Mouse Small Intestinal Immune System
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Here we describe a detailed protocol for the isolation of lymphocytes from the inductive sites including the gut-associated lymphoid tissue Peyer's patches and the draining mesenteric lymph nodes, and the effector sites including the lamina propria and the intestinal epithelium of the small intestinal immune...
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