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関連する概念動画

Microtubules01:35

Microtubules

98.2K
There are three types of cytoskeletal structures in eukaryotic cells—microfilaments, intermediate filaments, and microtubules. With a diameter of about 25 nm, microtubules are the thickest of these fibers. Microtubules carry out a variety of functions that include cell structure and support, transport of organelles, cell motility (movement), and the separation of chromosomes during cell division.
98.2K
Microtubules01:18

Microtubules

10.3K
Microtubules are the thickest cytoskeletal filaments with a diameter of 25 nm. In prokaryotic organisms, microtubules are commonly found in locomotory appendages like cilia and flagella. In eukaryotic cells, microtubules form specialized extensions for moving fluid over the surface, like those found in cells lining the intestine.
Microtubules have two structurally similar globular protein subunits: α and β tubulins. In the cytosol, the α and β tubulins form a heterodimer....
10.3K
Quantitative Microtubule Fractionation Technique to Separate Stable Microtubules, Labile Microtubules, and Free Tubulin in Mouse Tissues07:21

Quantitative Microtubule Fractionation Technique to Separate Stable Microtubules, Labile Microtubules, and Free Tubulin in Mouse Tissues

2.6K
Microtubules, which are tubulin polymers, play a crucial role as a cytoskeleton component in eukaryotic cells and are known for their dynamic instability. This study developed a method for fractionating microtubules to separate them into stable microtubules, labile microtubules, and free tubulin to evaluate the stability of microtubules in various mouse...
2.6K
Preparation of Segmented Microtubules to Study Motions Driven by the Disassembling Microtubule Ends12:20

Preparation of Segmented Microtubules to Study Motions Driven by the Disassembling Microtubule Ends

14.9K
Microtubules are inherently unstable polymers, and their switching between growth and shortening is stochastic and difficult to control. Here we describe protocols using segmented microtubules with photoablatable stabilizing caps. Depolymerization of segmented microtubules can be triggered with high temporal and spatial resolution, thereby assisting analysis of motions with the disassembling microtubule...
14.9K
Self-Assembly of Microtubule Tactoids08:49

Self-Assembly of Microtubule Tactoids

4.5K
This article presents a protocol for the formation of microtubule assemblies in the shape of tactoids using MAP65, a plant-based microtubule crosslinker, and PEG as a crowding...
4.5K
Extracting Modified Microtubules from Mammalian Cells to Study Microtubule-Protein Complexes by Cryo-Electron Microscopy08:02

Extracting Modified Microtubules from Mammalian Cells to Study Microtubule-Protein Complexes by Cryo-Electron Microscopy

1.9K
Here, we describe a protocol to extract endogenous tubulin from mammalian cells, which can lack or contain specific microtubule-modifying enzymes, to obtain microtubules enriched for a specific modification. We then describe how the extracted microtubules can be decorated with purified microtubule-binding proteins to prepare grids for cryo-electron...
1.9K

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関連する実験動画

Updated: Jan 19, 2026

Microtubules and Cell Motility
01:35

Microtubules and Cell Motility

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マイクロチューブル - ミエリーネーション 接続

Antonina Roll-Mecak1

  • 1Cell Biology and Biophysics Unit, National Institute of Neurological Disorders and Stroke, NIH, Bethesda, MD, USA; Biochemistry and Biophysics Center, National Heart, Lung and Blood Institute, NIH, Bethesda, MD, USA.

Cell
|September 17, 2019
PubMed
まとめ

研究者は,小管細胞の成長の重要なレギュラーを特定しました. このタンパク質は,ミエリンシート延伸と中枢神経系ミエリン化を制御して,ゴルギ前哨からの微小管の拡張を促進します.

科学分野:

  • 神経科学
  • 細胞生物学
  • 分子生物学

背景:

  • マイクロチューブルはオリゴデンドロサイトのプロセス拡張とミエリン沈殿に不可欠です.
  • オリゴデンドロサイトにおける微小管生殖の正確なメカニズムは,ほとんど不明である.

研究 の 目的:

  • オリゴデンドロサイトにおけるマイクロチューブルダイナミクスの新しい調節物質を特定する.
  • 骨髄膜形成と中枢神経の骨髄形成におけるこれらの調節体の役割を解明する.

主な方法:

  • オリゴデンドロサイト培養と操作
  • マイクロチューブルダイナミクス画像
  • ミエリンシート形成の分析 in vivoとin vitro

主要な成果:

  • オリゴデンドロサイトを濃縮した微小管調節体の特定
  • このレギュレータがゴルギの前哨からの微小管の成長を促進することを示す.
  • 骨髄膜の延長を制御する 制御装置の証拠

結論:

  • 特定されたレギュレータは,小胞細胞の微小管組織に不可欠です.

さらに関連する動画

Quantitative Microtubule Fractionation Technique to Separate Stable Microtubules, Labile Microtubules, and Free Tubulin in Mouse Tissues
07:21

Quantitative Microtubule Fractionation Technique to Separate Stable Microtubules, Labile Microtubules, and Free Tubulin in Mouse Tissues

Published on: November 17, 2023

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Preparation of Segmented Microtubules to Study Motions Driven by the Disassembling Microtubule Ends
12:20

Preparation of Segmented Microtubules to Study Motions Driven by the Disassembling Microtubule Ends

Published on: March 15, 2014

14.9K

関連する実験動画

Last Updated: Jan 19, 2026

Microtubules and Cell Motility
01:35

Microtubules and Cell Motility

98.2K
Quantitative Microtubule Fractionation Technique to Separate Stable Microtubules, Labile Microtubules, and Free Tubulin in Mouse Tissues
07:21

Quantitative Microtubule Fractionation Technique to Separate Stable Microtubules, Labile Microtubules, and Free Tubulin in Mouse Tissues

Published on: November 17, 2023

2.6K
Preparation of Segmented Microtubules to Study Motions Driven by the Disassembling Microtubule Ends
12:20

Preparation of Segmented Microtubules to Study Motions Driven by the Disassembling Microtubule Ends

Published on: March 15, 2014

14.9K
  • このタンパク質は,微小管の細胞構造と中枢神経の骨髄形成を結びつけるのに重要な役割を果たします.