生物材料からタンパク質を放出するための遺伝的にコードされた光分解性結合体
Jared A Shadish, Alder C Strange, Cole A DeForest1,2
1Department of Bioengineering , University of Washington , Seattle , Washington 98105 , United States.
Journal of the American Chemical Society
|September 19, 2019
まとめ
生物材料からタンパク質の放出を 光を用いて正確に制御する 新しい方法が開発されました このテクニックはタンパク質の活性を維持し,再生医療と診断における応用のためにパターンの付与を可能にします.
科学分野:
- バイオマテリアル科学
- バイオテクノロジー
- 分子生物学
背景:
- タンパク質は生物学的プロセスに不可欠ですが, 生物物質からタンパク質の生成と放出を制御することは依然として困難です.
- 現存する方法は,しばしば活動が低下した異質なタンパク質集団を生成し,成長因子のような脆弱な生物分子の応用を制限する.
研究 の 目的:
- 高精度でヒドロゲルから生物活性タンパク質を固定し,光放出するためのモジュラーでスケーラブルな方法を開発する.
- コントロールされた放出のための単一分散型 遺伝子コード化タンパク質キメラを作成します
主な方法:
- 化学酵素反応,バイオオートホグナル化学,および光遺伝学を用いて,光分解性タンパク質 (PhoCl) を介してタンパク質を水素ゲルに結合する.
- タンパク質の放出を空間時間的に制御するために,可視光 (λ ≈ 400 nm) を使ったマスクベースのレーザースキャニングリトグラフィーを採用した.
- 場所特有の不動化と,Focl結合器の用量依存の不可逆的な脊髄光分裂が実証された.
主要な成果:
- 保存された生物活性を持つ単分散タンパク質-ヒドロゲル結合体を成功裏に生成した.
- ヒドロゲルからタンパク質の放出を 空間時間的に正確に制御する.
- 3Dでアニゾトロプ的細胞増殖を促進する,光パターンの表皮成長因子のプレゼンテーションが実証された.
結論:
- 開発された方法は,生物材料からタンパク質の表示と放出を制御するためのスケーラブルで正確なアプローチを提供します.
- この技術はタンパク質の活性性を維持し パターンの出血を可能にし 診断や薬の出血や 再生医療の有望性を示しています
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