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Updated: Jan 19, 2026

An Assay for Quantifying Protein-RNA Binding in Bacteria
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An Assay for Quantifying Protein-RNA Binding in Bacteria

Published on: June 12, 2019

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バクテリアRNAポリメラーゼのデルタサブユニットの内在的に乱れた領域における機能的に重要な静電相互作用の定量構成分析

Vojtěch Kubáň, Pavel Srb1, Hana Štégnerová

  • 1Institute of Organic Chemistry and Biochemistry , Czech Academy of Sciences , v.v.i., Flemingovo nám 2 , Prague 6 16610 , Czech Republic.

Journal of the American Chemical Society
|September 26, 2019
PubMed
まとめ

高電荷の固有無秩序タンパク質 (IDP) は,静電相互作用により自体に折りたたまれます. この折り畳みは機能に不可欠で 変異がそれを破壊し 転写と細胞の健康に 影響を及ぼします

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Bacterial RNA Polymerase00:43

Bacterial RNA Polymerase

Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
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Bacterial Transcription

RNA polymerase (RNAP) carries out DNA-dependent RNA synthesis in both bacteria and eukaryotes. Bacteria do not have a membrane-bound nucleus. So, transcription and translation occur simultaneously, on the same DNA template.
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