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Updated: Jan 6, 2026

Inducible LAP-tagged Stable Cell Lines for Investigating Protein Function, Spatiotemporal Localization and Protein Interaction Networks
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Inducible LAP-tagged Stable Cell Lines for Investigating Protein Function, Spatiotemporal Localization and Protein Interaction Networks

Published on: December 24, 2016

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単一の再結合リガゼによって触媒化されたサイト固有の配列タンパク質ラベリング

Fabian B H Rehm1,2, Thibault J Harmand1, Kuok Yap2

  • 1Program in Cellular and Molecular Medicine , Boston Children's Hospital, Harvard Medical School , Boston , Massachussets 02115 , United States.

Journal of the American Chemical Society
|October 2, 2019
PubMed
まとめ

この研究では,オルデンランドアフィニスアスパラギニルエンドペプチダース (OaAEP1) を用いた新しいタンパク質結合法が導入されています. このアプローチにより,特定の部位で効率的で連続的なタンパク質ラベリングが可能になり,以前の方法の限界を克服します.

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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