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関連する概念動画

ATP Driven Pumps II: P-type Pumps01:34

ATP Driven Pumps II: P-type Pumps

6.0K
The P-type pumps are a large family of integral membrane transporter ATPases. They are divided into five major types based on substrate specificity, from I to V.
A typical P-type pump has three cytosolic domains: nucleotide-binding (N), phosphorylation (P), and activator (A) domains. These domains are connected to the membrane-spanning helices by short amino acid segments. ATP hydrolysis and covalent phosphoenzyme intermediate formation are crucial parts of the catalytic cycle. At the highly...
6.0K
ATP Driven Pumps III: V-type Pumps01:30

ATP Driven Pumps III: V-type Pumps

4.6K
V-type pumps are ATP-driven pumps found in the vacuolar membranes of plants, yeast, endosomal and lysosomal membranes of animal cells, plasma membranes of a few specialized eukaryotic cells, and some prokaryotes. They are also known as the V1Vo-ATPase, that couple ATP hydrolysis to transport protons against a concentration gradient.
The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...
4.6K
ATP Driven Pumps I: An Overview01:27

ATP Driven Pumps I: An Overview

9.6K
ATP-driven pumps, also known as transport ATPases, are integral membrane proteins. They have binding sites for ATP located on the membrane's cytosolic side and the ion-conducting domain in the transmembrane region. These pumps use the free energy released from ATP hydrolysis to move the solutes across cell membranes against an electrochemical gradient.
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and...
9.6K
Primary Active Transport01:29

Primary Active Transport

13.4K
In contrast to passive transport, active transport involves a substance being moved through membranes in a direction against its concentration or electrochemical gradient. There are two types of active transport: primary active transport and secondary active transport. Primary active transport utilizes chemical energy from ATP to drive protein pumps embedded in the cell membrane. With energy from ATP, the pumps transport ions against their electrochemical gradients—a direction they would...
13.4K
Primary Active Transport01:47

Primary Active Transport

195.6K
In contrast to passive transport, active transport involves a substance being moved through membranes in a direction against its concentration or electrochemical gradient. There are two types of active transport: primary active transport and secondary active transport. Primary active transport utilizes chemical energy from ATP to drive protein pumps that are embedded in the cell membrane. With energy from ATP, the pumps transport ions against their electrochemical gradients—a direction...
195.6K
Membrane Asymmetry Regulating Transporters01:19

Membrane Asymmetry Regulating Transporters

6.8K
Enzymes like flippase, floppase, and scramblase transfer phospholipids from one layer to another in the membrane, thereby affecting membrane asymmetry.
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...
6.8K

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関連する実験動画

Updated: Jan 5, 2026

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
11:55

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

Published on: August 16, 2016

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分子 の 二重 ポンプ

Yunyan Qiu1, Long Zhang1, Cristian Pezzato1

  • 1Department of Chemistry , Northwestern University , 2145 Sheridan Road , Evanston , Illinois 60208 , United States.

Journal of the American Chemical Society
|October 18, 2019
PubMed
まとめ
この要約は機械生成です。

研究者たちは エネルギーラッチメカニズムを使って 分子の動きを正確に制御する 分子二重ポンプ (MDP) を開発しました この人工分子の機械は 制御された捕獲と放出を可能にします 先進的な分子の輸送システムへの道を開きます

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High Speed Droplet-based Delivery System for Passive Pumping in Microfluidic Devices
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High Speed Droplet-based Delivery System for Passive Pumping in Microfluidic Devices

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Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies
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Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

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関連する実験動画

Last Updated: Jan 5, 2026

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
11:55

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

Published on: August 16, 2016

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High Speed Droplet-based Delivery System for Passive Pumping in Microfluidic Devices
10:22

High Speed Droplet-based Delivery System for Passive Pumping in Microfluidic Devices

Published on: September 2, 2009

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Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies
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科学分野:

  • 超分子化学
  • ナノテクノロジー
  • 化学工学

背景:

  • 人工分子機械 (AMM) は,制御された分子運動のためのエネルギーラッチを使用します.
  • 機械的に相互接続された分子 (MIM) がAMMの基礎となり,運動は運動障壁と熱力学的穴の変化によって制御される.
  • 以前の研究で,分子ダンベルに連続してリングをポンプする人工分子ポンプ (AMP) が確立されました.

研究 の 目的:

  • 2つのリンクされたAMPからなる新しい分子二重ポンプ (MDP) を報告する.
  • リドックス特性を使って,分子ダンベルをオン・オフする単一のリングの 線形制御されたポンプを実証する.
  • エネルギー・ラッチメカニズムで 分子を捕捉して放出するMDPの能力を示します

主な方法:

  • 2つの個々のAMPでヘッド・トゥ・テールリンクされたMDPの構築
  • ポンプ作用を制御するために,酸化還元特性を利用する.
  • 非共性相互作用と分子捕捉と放出のためのエネルギーラッチメカニズムを使用します.
  • 1Dと2D 1H NMRスペクトルで,片方向の動きと制御されたキャプチャ/リリースを監視する.

主要な成果:

  • MDPは分子ダンベルから単一のリングを 片方向で直線的にポンプで取り出すことに成功しました
  • リングの制御された捕獲と,その後の溶液への放出が実証された.
  • システムの機能は,NMRスペクトロスコーピーと制御実験によって検証されました.

結論:

  • 開発されたMDPは,分子輸送の正確な制御を証明する,AMMの重要な進歩を表しています.
  • この研究は,バクテリアホドプシンのような生物学的システムに類似した,より複雑な膜輸送能力を持つAMMの前駆者として機能します.
  • MDPは,将来の分子輸送プラットフォームの基礎を築き, プログラム可能な貨物の取り込みと放出機能を備えています.