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関連する概念動画

Positive Regulator Molecules01:45

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To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
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Circadian Rhythms and Gene Regulation02:19

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The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...
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Positive Regulator Molecules02:39

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Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
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M-Cdk Drives Transition Into Mitosis02:15

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Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
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Separation of Sister Chromatids02:17

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At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
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Anaphase Promoting Complex00:50

Anaphase Promoting Complex

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The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
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Updated: Apr 8, 2026

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
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Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

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MEL細胞のコミットメントとコルディセピンの同期

R Levenson, J Kernen, D Housman

    Cell
    |December 1, 1979
    PubMed
    まとめ

    コルディセピンは,ヌクレオチドアナログであり,ネズミの赤血球白血病 (MEL) 細胞における赤血球分化を阻害する. この発見は,細胞が端末分化へのコミットメントを制御する新しい分子機構を明らかにしています.

    科学分野:

    • * 分子生物学について
    • * 細胞の微分化
    • *がん研究について

    背景:

    • * マウス赤血球白血病 (MEL) 細胞は,赤血球分化の研究のためのモデルシステムです.
    • * 端末分化へのコミットメントには複雑な分子イベントが含まれています.
    • * ヌクレオチドアナログは,細胞のプロセスを調節することができます.

    研究 の 目的:

    • * コルディセピンがMEL細胞の分化に及ぼす影響を調査する.
    • * コルディセピンがエリソイド結合の分子イベントにおける役割を特定する.
    • * コルディセピンが特定の段階で分化を阻害する可能性を調査する.

    主な方法:

    • * 異なるMEL細胞の治療には,さまざまな用量のコルディセピンが必要です.
    • * 細胞結合と細胞毒性の評価.
    • * コルディセピンによる反転実験は,誘発剤の存在下で行われました.

    主要な成果:

    • * コルディセピンは,MEL細胞の染色体の分化へのコミットメントを迅速に抑制する.
    • * 抑制は,細胞毒性のない用量で発生する.
    • * コルディセピン治療の逆転により,迅速かつ同期的な細胞結合が可能になります.

    さらに関連する動画

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    Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
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    結論:

    • * コルディセピンは,以前は認識されなかったエリソイド分化コミットメントの側面を明らかにします.
    • * MEL細胞は,コルディセピンによって結合直前に止められる.
    • * コルディセピンは,細胞の運命決定の正確な分子タイミングを研究するためのツールを提供します.