合成 膀 から 膀 へ の 通信 システム
Yudi Ding1, Nicholas H Williams2, Christopher A Hunter1
1Department of Chemistry , University of Cambridge , Lensfield Road , Cambridge CB2 1EW , United Kingdom.
Journal of the American Chemical Society
|October 24, 2019
まとめ
研究者たちは 内部の触媒反応を誘発するために 膀を用いた新しい 分子信号システムを開発しました このシステムは 光出力を活性化するために 膀間のタンパク質転送を利用し 標的分子通信のための新しい方法を実証しています
科学分野:
- 生化学と分子生物学
- ナノテクノロジーと材料科学
- 化学工学
背景:
- 膀は細胞の伝達と信号伝達に不可欠です
- 膀の表面での分子相互作用を制御することは,合成生物学アプリケーションの鍵です.
- ターゲティングされたシグナル伝達経路を開発するには 精密な分子認識イベントが必要です
研究 の 目的:
- 細胞内触媒プロセスを誘発する 分子信号システムを設計する
- 制御された分子転移とシグナル生成のためにタンパク質-ベジクル相互作用を利用する.
- 特定の分子通信のための高親和結合システムを確立する.
主な方法:
- デシオビオチン-ニュートラヴィジン-バイオチン相互作用を用いて合成トランスデューサを膀二層に固定する.
- タンパク質の移転を誘導して 振動器を 膀の二重層に移動させる
- 光出力によるエステル水解のモニタリング,暴露した触媒ヘッドグループによって誘発される.
主要な成果:
- 一つの小胞の集団における 分子信号は 2番目の集団における 触媒的プロセスを成功裏に誘発した.
- NeutrAvidinの多価結合と高い親和性は,特定のタンパク質の転送とトランスデューサーのアンカリングを可能にしました.
- 標的膀に膜を固定したバイオチンは,効果的な信号伝達と触媒に不可欠でした.
結論:
- この研究は,小胞媒介による分子シグナル伝達と触媒の新しい方法を示しています.
- エンジニアリングされたシステムは 外部分子トリガーを介して細胞内反応を正確に制御できます
- この発見は 薬物投与,診断,合成生物学的システムに 潜在的に応用できます
関連する概念動画
Fusion of Secretory Vesicles with the Plasma Membrane
16.5K
Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
16.5K
Vesicular Tubular Clusters
3.0K
After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...
With the help of motor proteins such...
3.0K
Overview of Secretory Vesicles
9.3K
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
9.3K
SNAREs and Membrane Fusion
12.2K
Once a transport vesicle has recognized its target organelle, the vesicular membrane needs to fuse with the target membrane to unload the cargo. Transmembrane proteins called SNAREs present on organelle membranes and their vesicles, mediate vesicle fusion.
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
12.2K
Vesicular Trasport: Endocytosis, Transcytosis and Exocytosis
2.8K
Vesicular transport is a cellular process that encompasses the engulfment of particles or dissolved substances by cells. It involves endocytosis, transcytosis, and exocytosis.
Endocytosis is a cellular mechanism that involves the inward folding of the cell membrane to create vesicles that capture and transport large drug molecules. This process comprises two distinct methods: pinocytosis (often referred to as "cell drinking") and phagocytosis (often referred to as "cell...
Endocytosis is a cellular mechanism that involves the inward folding of the cell membrane to create vesicles that capture and transport large drug molecules. This process comprises two distinct methods: pinocytosis (often referred to as "cell drinking") and phagocytosis (often referred to as "cell...
2.8K
Clathrin Coated Vesicles
8.9K
Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
8.9K


