核素に結合したRSC複合体の構造
Youpi Ye1,2, Hao Wu2,3, Kangjing Chen1,2
1MOE Key Laboratory of Protein Science, Tsinghua University, Beijing 100084, P.R. China.
Science (New York, N.Y.)
|November 2, 2019
まとめ
RSCコンプレックス
科学分野:
- クロマチン生物学
- 遺伝子調節の分子機構
- 構造生物学
背景:
- RSC複合体は染色体の改造と遺伝子転写に不可欠です.
- RSCの構造を理解することは,DNAの調節におけるその機能を明らかにする鍵です.
- 関連するヒトの複合体の調節不良は癌に関与している.
研究 の 目的:
- 酵母RSC複合体の高解像度構造を決定する.
- RSCの染色体改造活動を制御する分子相互作用を解明する.
- RSCとそのヒトの同類体の機能についての洞察を提供するためです.
主な方法:
- クリオ電子顕微鏡 (cryo-EM) を用いて,RSC核細胞構造を可視化した.
- RSCの機能モジュールを定義するために生化学的および構造的分析が使用されました.
- ヒトの同位体との比較分析が行われました.
主要な成果:
- 構造は,RSCはATPアゼモーター,アクチン関連タンパク質モジュール,および基板募集モジュール (SRM) を含むことを示しています.
- RSCは主にそのモーターを通じて核胞子を結合し,Sfh1はH2A-H2B酸性パッチと相互作用することで核胞子の放出を促進する.
- SRMの組織と方向性は,DNA転位と核細胞の再定位を説明する.
- この研究では,RSCが核細胞のエピトープとどのように結合するかを詳細に説明しています.
結論:
- 決定された構造は,RSC媒介の染色体改造のメカニズム的理解を提供します.
- RSCの組立,機能,DNA転移の方向性を明らかにした.
- この研究は,BAF/PBAFとINI1/BAF47のような癌に関連したヒトの同類体を理解するための構造的枠組みを提供します.
関連する概念動画
02:10The Nucleosome Core Particle
14.0K
Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
The paradox
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their main responsibility is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. While on the other hand, they must allow polymerase enzymes to access DNA...
The paradox
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their main responsibility is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. While on the other hand, they must allow polymerase enzymes to access DNA...
14.0K
01:12The Nucleosome Core Particle
2.0K
Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their primary aim is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. On the other hand, they must allow polymerase enzymes to access histone-bound DNA during...
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their primary aim is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. On the other hand, they must allow polymerase enzymes to access histone-bound DNA during...
2.0K
02:33The Nucleosome
18.2K
DNA in a human cell is almost 2m long and it is packed inside a tiny nucleus that is only a few microns in diameter. The level of compaction of DNA inside the nucleus is astonishing. It is organized into several sequentially higher levels of compaction to fit into such a tiny space. The most compact form of DNA is a chromosome that can be seen under a microscope in a dividing cell.
DNA is wound twice around a protein complex called histone core, that consist of 8 histone proteins. This complex...
DNA is wound twice around a protein complex called histone core, that consist of 8 histone proteins. This complex...
18.2K
01:19The Nucleosome
3.5K
Human DNA is almost two meters long. However, it is compressed inside a tiny nucleus measuring only a few microns in diameter. To make this degree of compaction possible, DNA is organized into several sequential levels so that it can fit into such a tiny space. The most compact form of DNA is a chromosome that can be seen under a microscope in a dividing cell.
In a chromosome, DNA is wound twice around a protein complex called a histone octamer core, which consists of 8 histone proteins. This...
In a chromosome, DNA is wound twice around a protein complex called a histone octamer core, which consists of 8 histone proteins. This...
3.5K
02:33The Nucleosome
4.7K
4.7K
02:54Nucleosome Remodeling
10.7K
Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
10.7K