DNA複製中に活性化と抑制されたクロマチンの領域が明確な核分裂を示している

Thelma M Escobar¹, Ozgur Oksuz¹, Ricardo Saldaña-Meyer¹

¹Howard Hughes Medical Institute, New York University Langone Medical Center, New York, NY 10016, USA; New York University Langone Medical Center, New York, NY 10016, USA.

Cell

|November 2, 2019

まとめ

細胞分裂中に抑制されたクロマチンのドメインを保存するために,親核細胞は局所的に再埋蔵されます. この核細胞遺伝メカニズムは細胞のアイデンティティを維持し細胞の運命の変化に適応します

科学分野:

エピジェネティクスと分子生物学
細胞生物学
遺伝学

背景:

クロマチンドメインの忠実な遺伝は,細胞分裂を通して細胞のアイデンティティを維持するために不可欠です.
DNA複製の過程で親の核細胞とその改変がどのように移転されるかを理解することは,生きている細胞において困難です.

研究の目的:

DNA複製中に染色体領域の継承を保存するための局所的な戦略を調査する.
生体細胞におけるDNA複製後の親核細胞とその関連した翻訳後の改変 (PTM) の運命を追跡する.

主な方法:

ネズミの胚性幹細胞で誘導可能な,近接依存のラベリングシステムを開発した.
特定のゲノムロシオで不変に特徴づけられた複製依存のH3.1およびH3.2ヒストン核細胞.
DNA複製後の標識された親核細胞の分離と再収納をモニターした.

主要な成果:

抑制されたクロマチンのドメインは,親の核細胞の局所的な再積分によって保存されます.
活性クロマチンのドメインの核細胞は,親の核細胞と似た保存状態を示さない.
細胞の運命の変化は,親の核細胞遺伝を変化させ,染色体構造の相応のシフトを反映する.

結論:

両親の核細胞の局所的な再収納は,抑圧されたクロマチンの領域を維持するための重要なメカニズムである.
両親の核分裂パターンは細胞の運命の変化に適応し,クロマチンの構造と細胞のアイデンティティを保ちます
この研究は細胞記憶の基礎となる表遺伝的メカニズムに関する重要な洞察を明らかにしています

キーワード:

DNA複製エピジェネティック遺伝子発現遺伝子サイレンス親核細胞分離ポリコンブ翻訳後のヒストンの改変抑制されたクロマチン領域

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