ベータ・グロービン遺伝子スイッチングの発達調節

O R Choi¹, J D Engel

¹Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois 60201.

Cell

|October 7, 1988

まとめ

鶏のベータ・グロービン増強剤は通常,遺伝子発現を調節する. しかし,それは成人細胞の胚性エプシロン・グロービンを不適切に活性化させ,適切な遺伝子制御のための特定の規制要素の必要性を示している.

科学分野:

遺伝学遺伝学とは
分子生物学は分子生物学である.
発達生物学発達生物学について

背景:

成人ベータ遺伝子と胚性エプシロン・グロービン遺伝子の間に,鶏の赤血球細胞特異的な増強剤が存在する.
ベータグロービン増強剤は,両方のグロービン遺伝子の転写に影響を与えます.

研究の目的:

胚性および成人性グロービン遺伝子の双方のベータグロービン増強剤の調節作用を調査する.
染色体細胞における段階特異的な遺伝子調節のメカニズムを理解する.

主な方法:

削除および置換変異体の分析.
ベータおよびエプシロン・グロービン遺伝子の遺伝子発現を評価する.
プラズミドベースの遺伝子発現研究.

主要な成果:

ベータ・グロービン増強剤は,エプシロン遺伝子とベータ・グロービン遺伝子の両方の転写を刺激します.
エプシロン・グロービンは,ベータ・グロービン強化剤だけで調節されると,胚性および成人赤血球の両方で不適切に発現します.
ステージ特異的調節は,両方の遺伝子が単一のプラズミド上に存在するときに達成されます.
大人のベータ・グロービンのプロモーターには,発達段階セレクター要素 (SSE) が含まれています.

結論:

ベータグロービンの組織および発達段階特異的な調節は,成人ベータグロービンプロモーター内のベータグロービン強化剤とSSEの相互作用に依存します.
グロービン遺伝子発現の適切な調節には,適切な時間的および組織特異的な発現を確保するために,特定のプロモーター要素が必要です.

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