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古代のプロウイルスは,隣接するマウスの性制限タンパク質遺伝子にアンドロゲン調節を課した
1Department of Biological Sciences, Columbia University, New York, New York 10027.
Cell
|October 21, 1988
まとめ
マウスの性制限タンパク質 (Slp) 遺伝子の近くにある古代のプロウイルス挿入は,アンドロゲン調節を与えました. この長期の挿入変異は,遺伝子発現に影響を与える移植可能な要素の進化的維持を示しています.
科学分野:
- 遺伝学 遺伝学とは
- 分子生物学は分子生物学である.
- 進化生物学の進化生物学について
背景:
- ネズミの性制限タンパク質 (Slp) 遺伝子の発現はアンドロゲンに依存しており,その隣人,補完成分4 (C4) 遺伝子とは異なる.
- Slp と C4 の間の高い配列同一性は,共通の進化的起源を示唆しています.
研究 の 目的:
- Slp遺伝子のアンドロゲン依存的発現の背後にある規制メカニズムを調査する.
- Slp遺伝子にホルモン反応性を与える遺伝要素を特定する.
主な方法:
- SlpとC4遺伝子の比較配列分析. SlpとC4遺伝子の比較配列分析. SlpとC4遺伝子の比較配列分析.
- Slp遺伝子の上流に挿入された遺伝要素の識別と特徴付け.
- レトロウイルスのロング・ターミナル・リピート (LTR) 配列と変異の分析.
主要な成果:
- Slp遺伝子の約2kb上流で内生性プロウィルスが特定されました.
- このプロウイルスの5'LTRには,ホルモン反応性強化要素が含まれています.
- 配列の相違と突然変異は,プロウイルスが古くから存在し,マウスのゲノムに安定して統合されていることを示している.
結論:
- 古代のプロウイルス挿入は,隣接するSlp遺伝子にアンドロゲン反応性を授与しました.
- これは,転置可能な要素から進化的に維持される挿入変異の希少な例です.
- この発見は,宿主ゲノム調節と進化に対する内生レトロウイルスの長期的な影響についての洞察を提供します.
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