ステロイドホルモン受容体とその強化要素との分子相互作用:受容体二重体形成の証拠
S Y Tsai1, J Carlstedt-Duke, N L Weigel
1Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030.
Cell
|October 21, 1988
まとめ
ステロイドホルモン受容体は,グルココルチコイドおよびプロゲステロン受容体を含む,DNA応答要素に直接結合します. これらの受容体はDNAとダイマーとして相互作用し,協同結合のための特定の結合部位が特定されています.
科学分野:
- 分子生物学は分子生物学である.
- エンドクリノロジー エンドクリノロジー
- 遺伝学 遺伝学とは
背景:
- ステロイドホルモン受容体は,特定のDNA配列に結合することによって,遺伝子発現を調節する.
- グルココルチコイドとプロゲステロン受容体 (GR/PR) は,同様の反応要素 (GRE/PRE) を認識する.
研究 の 目的:
- ステロイドホルモン受容体とそのDNA応答要素の間の分子相互作用を調査する.
- GRE/PRE上のGR/PRの特定の結合部位と二酸化パターンを特定する.
主な方法:
- バンドシフトアッセイは,受容体-DNAの相互作用を研究するために使用されました.
- グルココルチコイド受容体のDNA結合ドメインのバクテリアのペプチド断片を使用した.
主要な成果:
- プロゲステロン受容体とグルココルチコイド受容体は,GRE/PREに特異的に結合する.
- 鶏のプロゲステロン受容体 (形AとB) とネズミのグルココルチコイド受容体では,同じピュリン接触部位が観察されました.
- GRE/PRE半部部位 (TGTTCTとTGTACA) に受容体ジマーの協力結合が実証されました.
結論:
- ステロイドホルモン受容体は,その同類の強化剤とダイマーとして相互作用する.
- DNA結合ドメインは,GRE/PREに特異的で協力的な結合を媒介する.
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