体細胞ハイブリッドにおける成長ホルモンの発現の消滅は,特定のトランス活性化剤GHF-1の抑制を伴う
A McCormick1, D Wu, J L Castrillo
1Department of Pharmacology, School of Medicine, University of California, San Diego, La Jolla 92093.
Cell
|October 21, 1988
まとめ
ハイブリッド細胞における成長ホルモン (GH) 生成の消滅は,抑制剤によるものではない. GH発現の喪失は,鍵となるトランスアクティベーターであるGHF-1の抑制に起因する.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- 細胞生物学 細胞生物学
背景:
- 成長ホルモン (GH) の発現は,GHプロモーターと結合するトランスアクティベーターGHF-1によって調節されます.
- ソマティック細胞のハイブリデーションは,典型的には,下垂体細胞におけるGHの産生を消し去り,転写抑制を示唆する.
研究 の 目的:
- フィブロブラスト x pituitary 細胞ハイブリッドにおける GH 発現の絶滅のメカニズムを解明する.
- GHF-1と潜在的抑制剤がGH遺伝子サイレンシングにおける役割を調査する.
主な方法:
- L細胞とGH3細胞の体細胞ハイブリッド化.
- イン・ビヴォの転移とイン・ビトロの転写アッセイ.
- タンパク質とmRNAのレベルを評価するために,DNAase Iの足跡と免疫ボットリング.
主要な成果:
- GH発現の絶滅は,3つのハイブリッド細胞系のうち2つの細胞系で観察されました.
- GH遺伝子の直接転写抑制剤の証拠は見つかりませんでした.
- GHF-1タンパク質とmRNA濃度の低下と相関するGH発現の減少.
結論:
- 線維芽細胞×下垂体ハイブリッドにおけるGH発現の絶滅は,GHF-1トランスアクティベーターの抑制によって媒介される.
- この抑制はGHF-1発現のレベルで起こりますが,GH遺伝子の直接的な沈黙によって起こることはありません.
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