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Updated: Jan 3, 2026

PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase
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PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase

Published on: September 26, 2020

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選択的四次炭素結合形成のためのトリプトファン合成 TrpB の調整

Markus Dick1, Nicholas S Sarai1, Michael W Martynowycz2

  • 1Division of Chemistry and Chemical Engineering 210-41 , California Institute of Technology , 1200 East California Boulevard , Pasadena , California 91125 , United States.

Journal of the American Chemical Society
|November 21, 2019
PubMed
まとめ

研究者は,新しい非正規のアミノ酸を作成するために,トリプトファン合成酵素の変種である TrpB Pf_quat を設計しました. この酵素は選択的に全炭素四分型ステレオセンターを形成し,合成化学の能力を拡張します.

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