Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

cAMP-dependent Protein Kinase Pathways01:25

cAMP-dependent Protein Kinase Pathways

8.1K
Cyclic Adenosine Monophosphate (cAMP) is an essential second messenger that activates protein kinase A (PKA) and regulates various biological processes. A single epinephrine molecule binds to GPCR and activates several heterotrimeric G proteins, each stimulating multiple adenylyl cyclase, amplifying the signal, and synthesizing large numbers of cAMP molecules. Small changes in cAMP concentration affect PKA activity. The binding of four cAMP molecules induces a conformational change in PKA,...
8.1K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

5.2K
The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
5.2K
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

11.6K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
11.6K
Protein Kinases and Phosphatases02:54

Protein Kinases and Phosphatases

14.7K
Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
14.7K
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

3.3K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
3.3K
What is Glycolysis?00:56

What is Glycolysis?

175.6K
Overview
Cells make energy by breaking down macromolecules. Cellular respiration is the biochemical process that converts "food energy" (from the chemical bonds of macromolecules) into chemical energy in the form of adenosine triphosphate (ATP). The first step of this tightly regulated and intricate process is glycolysis. The word glycolysis originates from the Latin glyco (sugar) and lysis (breakdown). Glycolysis serves two main intracellular functions: generating ATP and generating...
175.6K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Ku70-SAP domain has an overlapping function with DNA-PKcs in limiting the lateral movement of the Ku ring along DNA.

Nucleic acids research·2026
Same author

Decreasing Microtubule Detyrosination Improves Cardiac Mechanics and Sodium Channel Function in Arrhythmogenic Cardiomyopathy.

Circulation. Arrhythmia and electrophysiology·2026
Same author

Cuproptosis-immunity crosstalk informs strategy to overcome immunotherapy resistance.

Cell·2026
Same author

CD59 drives diet-induced obesity and glucose intolerance, insulin resistance, and metabolic dysfunction-associated steatotic liver disease.

npj metabolic health and disease·2026
Same author

Chemically induced skin tumors arise from long-lived stem cells of the upper hair follicle.

Science (New York, N.Y.)·2026
Same author

KRAS4A promotes oligomerization of hexokinase 1 on mitochondria.

Cell reports·2026

関連する実験動画

Updated: Jan 2, 2026

Identification of Kinase-substrate Pairs Using High Throughput Screening
11:13

Identification of Kinase-substrate Pairs Using High Throughput Screening

Published on: August 29, 2015

8.5K

KRAS4Aはヘクソキナーゼ1を直接調節する

Caroline R Amendola1, James P Mahaffey1, Seth J Parker1

  • 1Perlmutter Cancer Center, NYU School of Medicine, New York, NY, USA.

Nature
|December 13, 2019
PubMed
まとめ

KRAS4Aはヘクソキナーゼ1 (HK1) と直接相互作用し,その活性が変化する. この新しいKRAS4A-HK1相互作用は,KRASシグナル伝達と細胞代謝の間の直接的なリンクを明らかにし,がんにおける潜在的な治療目標を提供します.

さらに関連する動画

Study of the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2 Using Western Blotting
10:08

Study of the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2 Using Western Blotting

Published on: December 9, 2022

2.5K
Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

19.2K

関連する実験動画

Last Updated: Jan 2, 2026

Identification of Kinase-substrate Pairs Using High Throughput Screening
11:13

Identification of Kinase-substrate Pairs Using High Throughput Screening

Published on: August 29, 2015

8.5K
Study of the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2 Using Western Blotting
10:08

Study of the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2 Using Western Blotting

Published on: December 9, 2022

2.5K
Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

19.2K

科学分野:

  • 分子生物学
  • 腫瘍学
  • 細胞の代謝

背景:

  • KRASはがんにおいて頻繁に変異する腫瘍遺伝子であり,C末端部が異なるKRAS4AとKRAS4Bの同型を生成する.
  • 腫瘍性KRAS変異は細胞変異を活性化し,腫瘍細胞の代謝を変化させ,特にWarburg効果を誘発する.
  • 以前の研究では,直接的な酵素調節が不明のまま,転写の変化に代謝変化が起因していた.

研究 の 目的:

  • KRAS アイソフォームと代謝酵素の間の潜在的な直接の相互作用を調査する.
  • KRAS4Aが代謝酵素の活性を直接調節するかどうかを確認する.
  • KRAS4A媒介の代謝調節の機能的および治療的影響を探求する.

主な方法:

  • KRAS4Aとヘクソキナーゼ1 (HK1) の間のGTP依存の相互作用を検出するための生化学分析
  • 外部ミトコンドリア膜におけるKRAS4AとHK1のコロカライゼーションを調査する細胞局所化研究.
  • KRAS4A-HK1の相互作用がHK1の活動に与える影響を評価するための機能検査

主要な成果:

  • KRAS4Aとヘクソキナーゼ1 (HK1) の間には,GTPに依存する直接的な相互作用が確認された.
  • この相互作用は,HK1の酵素活性を直接変化させ,KRAS4AのエフェクタとしてHK1を確立することが示された.
  • KRAS4Aのユニークなパルミトイライレーションサイクルにより,外部のミトコンドリア膜にHK1とのコロカライゼーションが容易になります.

結論:

  • KRAS4AはHK1の活性を直接調節し,がんにおける新種の代謝制御メカニズムを表しています.
  • KRAS4A- HK1の相互作用は,がん代謝におけるKRASの異形特異的機能を強調する.
  • KRAS4A- HK1の相互作用をターゲットにすることで,KRAS4Aを発現するがんの独特の代謝の脆弱性を利用して,治療上の利益を得ることができます.