Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

RNA Splicing01:32

RNA Splicing

60.2K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
60.2K
Alternative RNA Splicing02:18

Alternative RNA Splicing

24.5K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
24.5K
Alternative RNA Splicing02:18

Alternative RNA Splicing

4.7K
4.7K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

8.0K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
8.0K
Pre-mRNA Processing: RNA Splicing01:36

Pre-mRNA Processing: RNA Splicing

6.4K
6.4K
pre-mRNA Processing02:01

pre-mRNA Processing

56.8K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
56.8K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Long-term feeder cell-free cat intestinal organoid cultures to study Toxoplasma gondii's sexual development.

Communications biology·2026
Same author

Publisher Correction: Polyamine-dependent metabolic shielding regulates alternative splicing.

Nature·2026
Same author

Polyamine-dependent metabolic shielding regulates alternative splicing.

Nature·2026
Same author

Unearthing soil biodiversity through collaborative genomic research and education.

Nature genetics·2025
Same author

A splice-switching antisense oligonucleotide approach for pediatric genetic epilepsies.

bioRxiv : the preprint server for biology·2025
Same author

AI-based HRCT quantification reveals DLCO and TLC as key determinants of ILD severity in connective tissue diseases.

RMD open·2025
Same journal

Whole-cell particle-based digital twin simulations from 4D lattice light-sheet microscopy data.

Cell·2026
Same journal

Systematic discovery of pathogen effector functions across human pathogens and pathways.

Cell·2026
Same journal

Structural basis for host membrane binding and remodeling by invading malaria parasites.

Cell·2026
Same journal

Multiscale integration of tissue and chromatin context converts cell heterogeneity into stable intestinal patterning.

Cell·2026
Same journal

Arc mediates intercellular tau transmission via extracellular vesicles.

Cell·2026
Same journal

Electromagnetic field-inducible in vivo gene switch for remote spatiotemporal control of gene expression.

Cell·2026
関連記事をすべて見る

関連する実験動画

Updated: Jan 2, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

5.3K

スプライシング コールバック

Tobias Hoffmann1, Juan Valcárcel2

  • 1Centre de Regulació Genòmica and Barcelona Institute of Science and Technology, Barcelona, Spain; Universitat Pompeu Fabra, Barcelona, Spain.

Cell
|December 14, 2019
PubMed
まとめ
この要約は機械生成です。

RNA前駆体からのイントロン除去は,しばしば共転写性である. 内部エクソンを活性化すると,代替の転写開始を促進し,遺伝子出力を高めることができます.

さらに関連する動画

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

3.1K
Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

9.3K

関連する実験動画

Last Updated: Jan 2, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

5.3K
A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

3.1K
Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

9.3K

科学分野:

  • 分子生物学
  • 遺伝学
  • 遺伝子規制

背景:

  • ユカリオットメッセンジャーRNA (mRNA) の前駆体からのイントロン除去は,遺伝子発現における重要なステップである.
  • このプロセスは,スプライシングとして知られており,転写 (共転写スプライシング) と同時に頻繁に発生します.
  • 代替スプライシングにより,1つの遺伝子が複数のタンパク質イソフォームを生成し,タンパク質の多様性が増加します.

研究 の 目的:

  • 遺伝子発現の調節における内部エクソン活性化の役割を調査する.
  • エクソン活性化によって代替の転写開始部位が影響されるかどうかを判断する.
  • 遺伝子発現の制御の基礎にある進化的および組織特異的なメカニズムを理解する.

主な方法:

  • 変異したエクソン活性化に対する遺伝子発現パターンの分析.
  • 代替の転写開始部位の特定と特徴付け
  • コトランスクリプションプロセスを研究するための計算的および実験的アプローチ.

主要な成果:

  • 進化的または組織特異的な内部エクソンの活性化が遺伝子発現を高めることが判明した.
  • この強化は,代替の転写開始部位の促進によって媒介されます.
  • この研究は,エクソン・スプライシングのダイナミクスを通じて遺伝子出力を調節するための新しいメカニズムを提供します.

結論:

  • 内部のエクソンの活性化は,遺伝子発現レベルを微調整するための規制メカニズムを表しています.
  • スプライシングとトランスクリプションの間の相互作用は 遺伝子制御の洗練された層を提供します
  • これらの発見は,発達と病気における遺伝子調節を理解するための意味を持つ.