関連する実験動画

Updated: Jan 2, 2026

In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells
08:58

In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells

Published on: May 1, 2019

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ポックスウイルス転写の構造的基礎: ワクチン複合体の転写と封鎖

Hauke S Hillen1, Julia Bartuli2, Clemens Grimm2

  • 1Department of Molecular Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany.

Cell
|December 14, 2019
PubMed
まとめ

ポックスウイルスは,ウイルスRNAポリメラーゼ (vRNAP) とキャピング酵素を用いてメッセンジャーRNA (mRNA) を合成する. この研究は,これらの複合体の冷凍電子顕微鏡構造を明らかにし,mRNA合成と改変を説明しています.

キーワード:
DNA依存型RNAポリメラーゼポックスウィリidaeRNAポリメラーゼワクチンキャピング酵素クリオ電子顕微鏡クリオ・エム延伸する構造トランスクリプション

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Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 1

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Viral Structure00:56

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RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
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