関連する実験動画

Updated: Jan 2, 2026

In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells
08:58

In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells

Published on: May 1, 2019

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ポックスウイルス転写の構造的基礎:ワクチンRNAポリメラーゼ複合体

Clemens Grimm1, Hauke S Hillen2, Kristina Bedenk1

  • 1Department of Biochemistry and Cancer Therapy Research Center (CTRC), Theodor Boveri-Institute, University of Würzburg, Am Hubland, 97074 Würzburg, Germany.

Cell
|December 14, 2019
PubMed
まとめ

Cryo-EM構造は,ワクチンウイルスRNAポリメラーゼ (vRNAP) とその転写因子を明らかにします. これらの発見は,宿主細胞内のウイルスの遺伝子発現とRNA処理のメカニズムを明らかにします.

キーワード:
DNA依存RNAポリメラーゼRNAポリメラーゼクリオ電子顕微鏡クリオ・エムクリオ・エム・再構築ポックスウィリidae単一の粒子構造トランスクリプションワクチン

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Transcription Initiation01:47

Transcription Initiation

Initiation is the first step of transcription in eukaryotes. Prokaryotic RNA Polymerase (RNAP) can bind to the template DNA and start transcribing. On the other hand, transcription in eukaryotes requires additional proteins, called transcription factors, to first bind to the promoter region in the DNA template. This binding helps recruit the specific RNAP that can assemble on the DNA and start transcription.
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Bacterial RNA Polymerase

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Eukaryotic RNA Polymerases00:58

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All three eukaryotic RNAPs require specific transcription factors, of which the...
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Bacterial Transcription

RNA polymerase (RNAP) carries out DNA-dependent RNA synthesis in both bacteria and eukaryotes. Bacteria do not have a membrane-bound nucleus. So, transcription and translation occur simultaneously, on the same DNA template.
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:
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