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関連する概念動画

Cohesins02:20

Cohesins

5.4K
Cohesin protein complexes are a molecular glue that holds two sister chromatids together. They play an important role both in mitosis and meiosis. In mitosis, all cohesin complexes present on the chromosomes are removed before the start of the anaphase stage.
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of...
5.4K
Attachment of Sister Chromatids02:57

Attachment of Sister Chromatids

3.9K
As cells progress into mitosis, the nuclear envelope breaks down, and the condensed chromosomes are exposed to the array of bipolar microtubules of the mitotic spindle. The kinetochore, a large, disc-shaped protein complex, is present at the centromere region of the sister chromatids and acts as a binding site for the microtubules.  Usually, the plus-end of a single microtubule is embedded within the kinetochore. However, some kinetochores first establish lateral contact with the side-wall...
3.9K
Structure of Cadherins01:25

Structure of Cadherins

4.5K
The cadherins were one of the first cell adhesion molecules discovered; the term “cadherins”   is based on their calcium-dependent adhering properties. The first cadherins discovered on the epithelial, neuronal, and placental cells were named E-cadherin, P-cadherin, and N-cadherin, respectively. These classical cadherins share sequence and structural similarities. Other cadherins, including those involved in cell signaling, are grouped into non-classical cadherins. This...
4.5K
Anchoring Junctions01:03

Anchoring Junctions

4.7K
Anchoring junctions are multiprotein complexes that help cells connect to other cells and the extracellular matrix. Anchoring junctions are present on the lateral and basal surfaces of cells, providing strong and flexible connections. Focal adhesions are often formed due to cell interactions with the ECM substrata, which initiate signal transduction via kinase cascades and other mechanisms. Together, they provide stability and tissue integrity. There are three types of anchoring junctions:...
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Cytoskeletal Accessory Proteins01:13

Cytoskeletal Accessory Proteins

3.8K
The cytoskeleton is an essential cell component that plays several structural and functional roles. However, the filaments that make up the cytoskeleton cannot function independently and depend on the accessory or ancillary proteins to effectively carry out their function. Accessory proteins associate with cytoskeletal filaments and their monomers, aiding filament formation and function. They also help in the cross-communication among cytoskeletal filaments. Cytoskeletal accessory proteins are...
3.8K
Desmosomes01:05

Desmosomes

7.1K
The term desmosome derives from the Greek words "desmo" and "soma" meaning "adhesion bodies." This structure was first observed during the late 1800s and described as small, dense nodules in the epidermis. Desmosomes are button-like structures that help form an interlinked network of intermediate filaments across the cells. These junctions are  essential to hold cells together under mechanical stress and to maintain tissue integrity. Desmosomes are multi-protein...
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関連する実験動画

Updated: Dec 31, 2025

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
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HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

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コヘシン-CTCF-アンカーされたループの構造的基礎

Yan Li1, Judith H I Haarhuis2, Ángela Sedeño Cacciatore2

  • 1European Molecular Biology Laboratory, Grenoble, France.

Nature
|January 7, 2020
PubMed
まとめ

コヘシンとCTCFタンパク質はループを形成してゲノムを構成する. この研究では,CTCFがコヘシンに直接結合し,ループ形成とゲノム組織を説明しています.

さらに関連する動画

In-Nucleus Hi-C in Drosophila Cells
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Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
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Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

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関連する実験動画

Last Updated: Dec 31, 2025

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

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In-Nucleus Hi-C in Drosophila Cells
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Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
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Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

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科学分野:

  • ゲノミクス
  • 分子生物学
  • 構造生物学

背景:

  • コヘシンとCTCFは,ゲノムがループに折り畳まれる際に重要なタンパク質です.
  • コヘシン-CTCF媒介による3Dゲノム構造化の正確な分子メカニズムは,まだ完全に理解されていません.

研究 の 目的:

  • CTCFとコヘシンとの相互作用の分子基盤を明らかにする.
  • この相互作用がCTCFで固定されたループ形成とコヘシンポジショニングにおける役割を理解する.

主な方法:

  • SA2-SCC1-CTCF複合体の構造を決定するX線結晶学.
  • 相互作用の機能的意義を検証する生化学的検査

主要な成果:

  • CTCFの特定のN端セグメントは,ヒトコヘシンのSA2-SCC1サブユニットと相互作用する.
  • 結晶構造は この相互作用の 分子詳細を明らかにします
  • この相互作用は,CTCFで固定されたループと結合部位におけるコヘシン局所化にとって極めて重要です.
  • 同様のモチーフは,WAPLを含む他のコヘシン結合体にも見られる.

結論:

  • CTCFはコヘシンをループの放出から保護し,それによってクロマチンループの形成を可能にします.
  • この研究は,コヘシンとCTCFによるゲノム折り畳みのダイナミックな調節に関する基本的な洞察を提供します.