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関連する概念動画

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

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Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
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Convolution Properties II01:17

Convolution Properties II

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The important convolution properties include width, area, differentiation, and integration properties.
The width property indicates that if the durations of input signals are T1 and T2, then the width of the output response equals the sum of both durations, irrespective of the shapes of the two functions. For instance, convolving two rectangular pulses with durations of 2 seconds and 1 second results in a function with a width of 3 seconds.
The area property asserts that the area under the...
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Deconvolution01:20

Deconvolution

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Deconvolution, also known as inverse filtering, is the process of extracting the impulse response from known input and output signals. This technique is vital in scenarios where the system's characteristics are unknown, and they must be inferred from the observable signals.
Deconvolution involves several mathematical techniques to derive the impulse response. One common approach is polynomial division. In this method, the input and output sequences are treated as coefficients of...
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Aliasing01:18

Aliasing

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Accurate signal sampling and reconstruction are crucial in various signal-processing applications. A time-domain signal's spectrum can be revealed using its Fourier transform. When this signal is sampled at a specific frequency, it results in multiple scaled replicas of the original spectrum in the frequency domain. The spacing of these replicas is determined by the sampling frequency.
If the sampling frequency is below the Nyquist rate, these replicas overlap, preventing the original...
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Upsampling01:22

Upsampling

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Managing signal sampling rates is essential in digital signal processing to maintain signal integrity. A decimated signal, characterized by a reduced frequency range due to its lower sampling rate, can be upsampled by inserting zeros between each sample. This upsampling process expands the original spectrum and introduces repeated spectral replicas at intervals dictated by the new Nyquist frequency. To refine this zero-inserted sequence, it is passed through a lowpass filter with a cutoff...
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Vivoアップコンバージョンイメージングにおけるタイムマルチプレックス

Hui Li1, Meiling Tan1, Xin Wang1

  • 1MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, School of Chemistry and Chemical Engineering & Key Laboratory of Micro-systems and Micro-structures, Ministry of Education , Harbin Institute of Technology , Harbin 150001 , People's Republic of China.

Journal of the American Chemical Society
|January 8, 2020
PubMed
まとめ
この要約は機械生成です。

新しいアップコンバージョンナノ粒子 (UCNPs) は,異なる発光寿命を使用して,多重化 in vivo イメージングを可能にします. この画期的な発見により 組織の深部で高コントラストで 多色画像を撮ることができ バイオ画像と診断を 進歩させました

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科学分野:

  • ナノテクノロジー
  • 生物医学工学
  • 材料科学

背景:

  • アップコンバージョンナノ粒子 (UCNP) は,近赤外線 (NIR) 光を可視光に変換し,バイオイメージングに不可欠です.
  • マルチプレキスされた in vivo 画像は,深部組織に浸透するUCNP 発光帯の希少性によって制限されています.

研究 の 目的:

  • タイム・ドメイン・ディスクリミネーションを用いたマルチプレックスアップコンバージョンのイン・ビボ画像の新しいアプローチの開発.
  • 既存のUCNP発光帯の限界を克服し,深層組織に浸透する.

主な方法:

  • エネルギー移転とアップコンバージョンプロセスを制御するためのテトラドメインナノ構造を設計した.
  • 異なる寿命を持つNIR発光 (808 nm) の時間領域差別を使用した.
  • クンミンマウスに静脈内および皮下投与した水性UCNP.

主要な成果:

  • 精密に制御された寿命で2桁の大きさ (782157 μs) をカバーする高量子収量アップ変換発光 (最大約6.1%) を達成した.
  • マウス肝臓と皮下組織におけるUCNPの高コントラスト,生涯カラーイメージングが実証された.
  • 光学パターンと定義された寿命を使用して解読された画像の多色表示を有効にします.

結論:

  • テンポラルマルチプレックスアップコンバージョンのアプローチは,先端の in vivo 画像と偽造防止を可能にします.
  • この方法は,高通量バイオセンシング,体積表示,診断および治療に重要な意味を持っています.