ヒトのグルココルチコイド受容体の複数の協力的なトランス活性化ドメイン
1Howard Hughes Medical Institute, Salk Institute for Biological Studies, La Jolla, California.
Cell
|December 2, 1988
まとめ
研究者らは,ヒトのグルココルチコイド受容体 (hGR) 内の2つの活性化ドメインを特定しました. これらの配列,タウ2とアミノ端にある別の配列は,受容体の機能とホルモンの誘導性にとって極めて重要です.
科学分野:
- 分子生物学は分子生物学である.
- エンドクリノロジー エンドクリノロジー
- 遺伝学 遺伝学とは
背景:
- 人間のグルココルチコイド受容体 (hGR) は,遺伝子発現を調節する重要な核受容体です.
- アクティベーションドメインは,核受容体の転写活動に不可欠です.
研究 の 目的:
- hGR内の機能的アクティベーションドメインを特定し,特徴づけること.
- これらの活性化ドメインの性質と局所を調査する.
主な方法:
- 酵母 GAL4融合タンパク質を用いたペプチドマッピングと機能分析.
- 配列特性と機能的モジュール性の分析.
主要な成果:
- hGRのカルボキシル末端にある30アミノ酸ペプチド (tau2) は,強力な活性化ドメインとして機能する.
- タウ2は,GAL4と融合すると,ホルモン誘導の転写活性を与える.
- hGRのアミノ末端に第二の独立した活性化ドメインが特定されました.
- 両ドメインも酸性であり,位置にかかわらず機能する.
結論:
- hGRには少なくとも2つの異なる活性化ドメインが含まれており,一つはカルボキシル末端 (tau2) で,もう一つはアミノ末端にあります.
- これらのドメインは,ホルモン誘導転写因子としてのhGRの役割に不可欠です.
- これらのドメインの酸性性は,酵母活性化剤配列と共通する特徴かもしれません.
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