制御された自己燃焼によるシーケンス定義オリゴウレタンのシーケンス化

Samuel D Dahlhauser¹, P Rogelio Escamilla¹, Abigail N VandeWalle¹

¹Department of Chemistry , The University of Texas at Austin , Austin , Texas 78712 , United States.

Journal of the American Chemical Society

|January 28, 2020

まとめ

研究者は,自己燃焼プロセスを用いてオリゴウレタンとポリマーをシーケンスする方法を開発しました. この技術は,質量スペクトロメトリーなしで複合分子をモノマーに"解き放つ"ことで素早く特徴づけます.

科学分野:

ポリマー化学
有機合成
材料科学

背景:

配列で定義されたポリマーはバイオミメティクス,自己組み立て,触媒,情報保存などの応用に不可欠です
複雑な化学機能を解き放つにはポリマーの基本構造を正確に制御することが重要です

研究の目的:

離散型オリゴウレタンの効率的な溶液相および固体相合成方法の開発.
オリゴウレタンとポリマーの迅速かつ堅固な特徴づけのための自己燃焼性シーケンシング戦略を確立する.

主な方法:

溶液相と高収量固体相の両方を用いて合成された離散性オリゴウレタン.
制御された,反復的な反応を開始するために,端末アルコールグループの固有の反応性を利用
解錠する
自分を燃やすプロセスです
液体染色体質スペクトロメトリー (LC/MS) を用いて自己燃焼反応をモニターし,配列決定のためのアルゴリズムを適用した.

主要な成果:

分離性オリゴウレタンの高収量合成を達成した.
MS/MSのないオリゴメアを素早く特徴づける新しい自己燃焼性シーケンシング方法を示した.
解凍過程で2オクサゾリジノンとして解き放たれた各モノマーを成功裏に識別した.
適用されたアルゴリズムは,LC/MSデータからオリゴレタン配列を正確に推論した.

結論:

開発された自己燃焼シーケンスメソッドは,オリゴウレタンおよび関連するポリマーの迅速かつ堅固な特徴づけを提供します.
このアプローチは,ポリマー配列にコードされた分子情報を効果的に読み取り,複雑な構造の特徴づけを可能にします.
この方法論は,配列定義ポリマーを分析するための強力なツールであり,さまざまな分野でその応用を進めています.

さらに関連する動画

13:42

Solid-phase Submonomer Synthesis of Peptoid Polymers and their Self-Assembly into Highly-Ordered Nanosheets

Published on: November 2, 2011

30.0K

05:15

Solid-phase Synthesis of [4.4] Spirocyclic Oximes

Published on: February 6, 2019

7.2K

関連する概念動画

01:05

Maxam-Gilbert Sequencing

12.4K

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...

12.4K

03:00

Next-generation Sequencing

97.4K

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....

97.4K

00:53

Olefin Metathesis Polymerization: Acyclic Diene Metathesis (ADMET)

2.1K

Acyclic diene metathesis polymerization or ADMET polymerization involves cross-metathesis of terminal dienes, such as 1,8-nonadiene, to give linear unsaturated polymer and ethylene. As ADMET is a reversible process, the formed ethylene gas must be removed from the reaction mixture to complete the polymerization process.
Similar to cross-metathesis, ADMET also involves the formation of metallacyclobutane intermediate by [2+2] cycloaddition of one of the double bonds of a terminal diene with...

2.1K

01:16

Olefin Metathesis Polymerization: Ring-Opening Metathesis Polymerization (ROMP)

3.0K

Ring-opening metathesis polymerization or ROMP involves strained cycloalkenes as starting materials. The mechanism of ROMP proceeds by reacting cycloalkene with Grubbs catalyst to give metallacyclobutane intermediate which undergoes a ring-opening reaction to form new carbene. The new carbene reacts with another molecule of cycloalkene. Repetition of these steps leads to the formation of an unsaturated open-chain polymer product. All these steps are reversible, however, relieving the ring...

3.0K

01:57

Sanger Sequencing

772.4K

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

772.4K

03:00

Preparation of Epoxides

9.0K

Overview
Epoxides result from alkene oxidation, which can be achieved by a) air, b) peroxy acids, c) hypochlorous acids, and d) halohydrin cyclization.
Epoxidation with Peroxy Acids
Epoxidation of alkenes via oxidation with peroxy acids involves the conversion of a carbon–carbon double bond to an epoxide using the oxidizing agent meta-chloroperoxybenzoic acid, commonly known as MCPBA. Since the O–O bond of peroxy acids is very weak, the addition of electrophilic oxygen of peroxy acids to...