argFとargl遺伝子の進化的ドリフトである. E. coli K12におけるオルニチン・トランスカルバミラーゼのイソエンザイム形態のコーディング
Cell
|February 1, 1977
まとめ
遺伝的ドリフトは,E. coli K12のargFとargl遺伝子を著しく変えた. オルニチントランスカルバミラーゼをコードするこれらの遺伝子は,祖先の遺伝子から25-40%の塩基対の逸脱を示しています.
科学分野:
- 微生物学 微生物学とは
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
背景:
- argFおよびargl遺伝子は,E. coli K12におけるオルニチントランスカルバミラーゼの異なった異酵素形態をコードする.
- 遺伝子の進化と分岐を理解することは,微生物遺伝学にとって極めて重要です.
研究 の 目的:
- E. coli K12のargFとargl遺伝子の間の遺伝的差異を定量化するために.
- これらの特定の遺伝子に対する遺伝的ドリフトの影響を評価する.
主な方法:
- mRNA-DNAのハイブリッド化技術を利用した.
- ハイブリダイゼーションプローブとしてアルギニン変換DNAを使用した.
- 特殊な変換ファグ (gammacI857darglとphi80dargF) のDNAを用いてインビトロで合成されたmRNA.
主要な成果:
- 塩基対の25-40%が,その分岐以来,argFとarglの両方の遺伝子で変化したことを決定しました.
- 2つのイソ酵素遺伝子の間の定量化された実質的な遺伝的漂移.
結論:
- argFとargl遺伝子は,遺伝的漂移により,進化的に大きく変化しています.
- この研究は,これらの重要な代謝遺伝子の分岐の定量的な尺度を提供します.
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