タンパク質を含み,提供する水溶性柔軟な有機構造
Jia-Le Lin1, Ze-Kun Wang1, Zi-Yue Xu1
1Department of Chemistry, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials , Fudan University , 2205 Songhu Road , Shanghai 200438 , China.
Journal of the American Chemical Society
|February 4, 2020
まとめ
新しい水溶性有機フレームワーク (FOF-1-4) はタンパク質を細胞に効率的に供給します. これらの柔軟で多孔な材料は 細胞毒性が低く 生物分子の負荷能力が高く 細胞の有効な吸収を可能にします
科学分野:
- 材料科学
- 超分子化学
- ナノテクノロジー
背景:
- 薬剤の投与のための新しい多孔性の材料の開発.
- 水溶性で生物互換性のあるナノキャリアの必要性
- タンパク質の効率的な細胞内配送の課題
研究 の 目的:
- 水溶性ヒドラゾン基の新型有機フレームワーク (FOF-1-4) を合成し,特徴づけること.
- これらのフレームワークの毛細さと負荷能力を調査する.
- 細胞にタンパク質を供給する可能性を評価する.
主な方法:
- テトラケーション性テトラアルデヒドと柔軟な二水化物を用いたFOF-1-4の合成
- 1H NMRスペクトロスコーピーとダイナミック光分散を用いた特徴付け.
- エタノール蒸気吸収とゲスト分子の負荷による孔性の評価
- フローサイトメトリーを用いたインビトロタンパク質配分試験.
主要な成果:
- 4つの水溶性 柔軟な有機フレームワーク (FOF-1-4) の合成に成功しました
- フレームワークは50~120nmの水力ダイナミック直径と5.3~6.7nmの固有の孔径を示しています.
- ポルフィリンゲストに対する高い負荷能力とタンパク質の急速な含有 (BSA,GFP,OFP)
- タンパク質を正常な細胞とがん細胞に効率的に送り込み,最大99. 8%の細胞伝達率を達成した.
結論:
- FOF-1-4は水溶性多孔な有機フレームワークの新種です
- これらの材料は優れた生物互換性と 効率的なタンパク質包装と配送能力を示しています
- 開発されたフレームワークは,先進的な薬剤とタンパク質の配送アプリケーションにとって非常に有望です.
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