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H2/α-ケト酸酵素燃料電池におけるN2からキラルアミノ酸へのバイオエレクトロカタリティック変換
Hui Chen1, Matthew B Prater1, Rong Cai1
1Department of Chemistry , University of Utah , 315 South 1400 East, Room 2020 , Salt Lake City , Utah 84112 , United States.
Journal of the American Chemical Society
|February 5, 2020
まとめ
この研究では,窒素ガスをキラルアミノ酸に変換する酵素燃料電池 (EFC) が開発されました. この自給自足システムは 価値ある生化学薬品の生産に エネルギー効率の良い方法を提供します
科学分野:
- バイオテクノロジー
- 生物触媒
- 緑の化学
背景:
- 酵素による電気合成は 電気エネルギーを用いた化学生産の 持続可能な経路を提供します
- 酵素燃料電池は化学エネルギーを電気エネルギーに変換し,自己動力反応を可能にします.
- 電気合成とEFCを統合することで,高価値の化学物質を効率的かつ自律的に生産することができます.
研究 の 目的:
- 不活性な窒素ガスをキラルアミノ酸に変換するための水素/α-ケト酸EFCを開発する.
- アンモニアと2-ケトヘキサノ酸からL-ノルルエシンを現地で生産するための自給システムを作成する.
- バイオカタリシスを用いてキラルアミノ酸を生産するためのエネルギー効率の良い代替案を実証する.
主な方法:
- アンモニアとNADHの生成のための窒素酵素とダイアフォラゼを含むカソド反応カスケードを設計し,構築した.
- レウシン脱水素酵素 (LeuDH) を利用して,l-ノルルシンを in situ 合成した.
- 水素燃料電池と超熱安定ヒドロゲナーゼ (SHI) アノドとカソッドカスケードを統合してH2/α-ケト酸EFCを形成した.
主要な成果:
- 92%のアンモニア変換と87.1%のファラダイク効率をキャソードカスケードで達成した.
- H2/α-ケト酸EFCは10時間後に99%以上のエナティオメール過剰で0.36mMのl-ノルルエシンを生成した.
- 統合されたEFCシステムでl-ノルルエシン生産の82%のファラダイク効率を達成した.
結論:
- 開発されたH2/α-ケト酸EFCは,キラルアミノ酸を生産するための実行可能でエネルギー効率の高い方法である.
- このシステムは,生物工学の応用のための LeuDH の広範な基板範囲と高いエナチオ選択性を活用しています.
- このアプローチは,キラルアミノ酸合成のための伝統的な電気化学的方法の持続可能な代替案を提供します.
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