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関連する概念動画

Sanger Sequencing01:57

Sanger Sequencing

772.3K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
772.3K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

12.4K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
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Next-generation Sequencing03:00

Next-generation Sequencing

97.3K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
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RNA-seq03:21

RNA-seq

11.6K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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関連する実験動画

Updated: Dec 28, 2025

Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

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DNA 5-メチルサイトシン特異増幅と配列決定

Chang Liu1,2, Xiaolong Cui1,2, Boxuan Simen Zhao3

  • 1Department of Chemistry, Department of Biochemistry and Molecular Biology, and Institute for Biophysical Dynamics, The University of Chicago, Chicago, Illinois 60637, United States.

Journal of the American Chemical Society
|February 21, 2020
PubMed
まとめ

この研究では,精密なDNA5メチルサイトシン (5mC) マッピングのための新しい全ゲノム増幅方法が導入されています. この新しい技術は,DNAの最小限から5mCを保持し,以前の方法の限界を克服します.

さらに関連する動画

Selective Capture of 5-hydroxymethylcytosine from Genomic DNA
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Selective Capture of 5-hydroxymethylcytosine from Genomic DNA

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High Sensitivity 5-hydroxymethylcytosine Detection in Balb/C Brain Tissue
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High Sensitivity 5-hydroxymethylcytosine Detection in Balb/C Brain Tissue

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関連する実験動画

Last Updated: Dec 28, 2025

Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

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Selective Capture of 5-hydroxymethylcytosine from Genomic DNA
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Selective Capture of 5-hydroxymethylcytosine from Genomic DNA

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High Sensitivity 5-hydroxymethylcytosine Detection in Balb/C Brain Tissue
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High Sensitivity 5-hydroxymethylcytosine Detection in Balb/C Brain Tissue

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科学分野:

  • エピジェネティクス
  • 分子生物学
  • ゲノミクス

背景:

  • DNA5メチルサイトシン (5mC) マッピングのための従来のビスルフィート配列は,DNAの分解に苦しんでいます.
  • 5ヒドロキシメチルサイトシン (5hmC) の存在は,標準的な方法を使用して正確な5mC検出を困難にします.

研究 の 目的:

  • 5-メチルサイトシン (5mC) を高精度で特定するDNA増幅方法を開発する.
  • 5mCメチローム解析におけるDNA分解と5hmC干渉の限界を克服するために.

主な方法:

  • 新しい5mC特異的な全ゲノム増幅技術 (5mC-WGA) が開発されました.
  • この方法はDNAの入力量を10 pgに最適化しました.
  • 5hmC信号からの5mC保持と干渉の評価が行われました.

主要な成果:

  • 5mC-WGA方法はDNA増幅中に5mC信号を成功裏に保持しました.
  • 5ヒドロキシメチルサイトシン (5hmC) 信号からの干渉は最小限であった.
  • DNA 5mCメチロームプロファイルの高い再現性と精度が達成されました.

結論:

  • 開発された5mC-WGA方法は,正確なDNA5mCメチローム分析のための堅固な解決策を提供します.
  • この技術は,限られたDNA入力でも信頼性の高い表遺伝子研究を可能にします.
  • 従来のアプローチと比較して,5mCマッピングの精度を大幅に改善します.